Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaCl | 1 M, activation of enzyme form IAPase | Serratia marcescens |
General Stability | Organism |
---|---|
Mg2+ protects against thermal inactivation | Serratia marcescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | 1 M, inhibition of enzyme form CAPase | Serratia marcescens | |
phosphate | competitive inhibition of both enzyme forms | Serratia marcescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0083 | - |
4-nitrophenyl phosphate | IAPase | Serratia marcescens | |
0.057 | - |
4-nitrophenyl phosphate | CAPase | Serratia marcescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | - |
2 distinct enzyme forms, an inducible enzyme form IAPase in 4 isozymes, and a constitutive enzyme form CAPase | - |
Serratia marcescens 211 | - |
2 distinct enzyme forms, an inducible enzyme form IAPase in 4 isozymes, and a constitutive enzyme form CAPase | - |
Purification (Comment) | Organism |
---|---|
2 distinct enzyme forms, CAPase 212fold and 4 isozymes of IAPase 5-87fold | Serratia marcescens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a phosphate monoester + H2O = an alcohol + phosphate | Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate | Serratia marcescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.5 | - |
purified isozyme IV of IAPase | Serratia marcescens |
22.2 | - |
purified isozyme III of IAPase | Serratia marcescens |
30.8 | - |
purified isozyme I of IAPase | Serratia marcescens |
43.5 | - |
purified isozyme II of IAPase | Serratia marcescens |
106 | - |
purified CAPase | Serratia marcescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Serratia marcescens | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Serratia marcescens 211 | 4-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alkaline phosphatase | - |
Serratia marcescens |
APase | - |
Serratia marcescens |
CAPase | - |
Serratia marcescens |
IAPase | - |
Serratia marcescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Mg2+ protects against thermal inactivation up to 80°C | Serratia marcescens |
95 | - |
5 min, in absence or presence of 10 mM Mg2+, inactivation of CAPase, while IApase is relatively stable showing loss of 30% activity after 5 min and loss of 80% activity after 30 min | Serratia marcescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10 | IAPase | Serratia marcescens |
9.5 | - |
CAPase | Serratia marcescens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0053 | - |
phosphate | IAPase | Serratia marcescens | |
0.76 | - |
phosphate | CAPase | Serratia marcescens |