Any feedback?
Literature summary for 3.1.26.7 extracted from
- Helix P4 is a divalent metal ion binding site in the conserved core of the ribonuclease P ribozyme (2000), RNA, 6, 511-519.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of 2 self-cleaving ribozyme-substrate conjugates for identification of nucleotides involved in catalysis | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | effects of phosphorothioate substitutions on ribozyme catalysis, kinetics, overview | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 25 mM, required for folding and catalytic function, metal binding site and coordination study using site-specific phosphorothioate substitutions, helix P4: the pro-Sp phosphate of Ala67 is involved in metal ion positioning | Escherichia coli |  |
| NaCl | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pre-tRNA + H2O | Escherichia coli | the helix P4 is important in the ribozyme for positioning divalent metal ions that are required for catalysis | tRNA + pre-part nucleotides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pre-tRNA + H2O | - |
Escherichia coli | tRNA + pre-part nucleotides | - |
? | |
| pre-tRNA + H2O | the helix P4 is important in the ribozyme for positioning divalent metal ions that are required for catalysis | Escherichia coli | tRNA + pre-part nucleotides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | ribozyme secondary structures | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribonuclease P ribozyme | - |
Escherichia coli |
| RNase P RNA | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
