Literature summary for 3.1.26.5 extracted from

Kouzuma, Y.; Mizoguchi, M.; Takagi, H.; Fukuhara, H.; Tsukamoto, M.; Numata, T.; Kimura, M.
Reconstitution of archaeal ribonuclease P from RNA and four protein components (2003), Biochem. Biophys. Res. Commun., 306, 666-673 .

Search for more literature for 3.1.26.5

Cloned(Commentary)

Cloned (Comment)	Organism
ribonuclease P protein components 1, 2, 3 and 4 are expressed in Escherichia coli cells, and the resulting proteins Ph1481p, Ph1601p, Ph1771p, and Ph1877p are purified to apparent homogeneity in a set of column chromatographies. RNase P reconstituted of four proteins with the in vitro transcripted Pyrococcus horikoshii RNase P RNA exhibits enzymatic properties like those of native enzyme	Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
pre-tRNA + H2O	Pyrococcus horikoshii	-	mature tRNA + 5'-terminal oligonucleotide	-	?

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	O59425 and O59150 and O59543 and O59248 and P62009	O59425: ribonuclease P protein component 1 (PhoRpp29), O59150: ribonuclease P protein component 2 (PhoPop5), O59543: ribonuclease P protein component 3 (PhoRpp30), O59248: ribonuclease P protein component 4 (RPP21), 50S ribosomal protein L7Ae (PhoRpp38). RNase P consists of a catalytic RNase P RNA (PhopRNA) and five protein cofactors designated PhoPop5, PhoRpp21, PhoRpp29, PhoRpp30, and PhoRpp38	-

Purification (Commentary)

Purification (Comment)	Organism
ribonuclease P protein components 1, 2, 3 and 4 are expressed in Escherichia coli cells, and the resulting proteins Ph1481p, Ph1601p, Ph1771p, and Ph1877p are purified to apparent homogeneity in a set of column chromatographies. RNase P reconstituted of four proteins with the in vitro transcripted Pyrococcus horikoshii RNase P RNA exhibits enzymatic properties like those of native enzyme	Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pre-tRNA + H2O	-	Pyrococcus horikoshii	mature tRNA + 5'-terminal oligonucleotide	-	?
pre-tRNA(Tyr) + H2O	-	Pyrococcus horikoshii	mature tRNA(Tyr) + 5'-terminal oligonucleotide	-	?

Subunits

Subunits	Comment	Organism
tetramer	ribonuclease P protein component 1 (PH1771), ribonuclease P protein component 2 (PH1481), ribonuclease P protein component 3 (PH1877), ribonuclease P protein component 4 (PH1601). Three proteins Ph1481p, Ph1601p, and Ph1771p, and RNase P RNA are minimal components for the RNase P activity. However, addition of the fourth protein Ph1877p strongly stimulated enzymatic activity, indicating that all four proteins and RNase P RNA are essential for optimal RNase P activity	Pyrococcus horikoshii

Synonyms

Synonyms	Comment	Organism
RNase P	-	Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	-	assay at	Pyrococcus horikoshii

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Release 2023.1 (January 2023)