Cloned (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
- |
Pyrococcus furiosus |
- |
Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Methanothermobacter thermautotrophicus | - |
- |
- |
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
- |
Pyrococcus furiosus |
- |
Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNATyr + H2O | pre-tRNATyr from Escherichia coli | Methanothermobacter thermautotrophicus | tRNATyr + 5'-oligoribonucleotide | - |
? | |
pre-tRNATyr + H2O | pre-tRNATyr from Escherichia coli | Pyrococcus furiosus | tRNATyr + 5'-oligoribonucleotide | - |
? | |
pre-tRNATyr + H2O | pre-tRNATyr from Escherichia coli | Methanocaldococcus jannaschii | tRNATyr + 5'-oligoribonucleotide | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | the archaeal holoenzyme is associated with 1 RNase P RNA and at least 4 RNase P proteins (POP5, RPP30, RPP21 and RPP29). Archaeal RNase P proteins function as two binary RNase P protein complexes (POP5/RPP30 and RPP21/RPP29). Archaeal POP5/RPP30 reconstituted with bacterial and organellar RNase P RNAs. While POP5/RPP30 is solely responsible for enhancing the cleavage rate of precursor tRNA by RNase P RNAs (by 60fold), RPP21/RPP29 contributes to increased substrate affinity (by 16-fold) | Pyrococcus furiosus |
oligomer | the archaeal holoenzyme is associated with 1 RNase P RNA and at least 4 RNase P proteins (POP5, RPP30, RPP21 and RPP29). Archaeal RNase P proteins function as two binary RNase P protein complexes (POP5/RPP30 and RPP21/RPP29). Archaeal POP5/RPP30 reconstituted with bacterial and organellar RNase P RNAs. While POP5/RPP30 is solely responsible for enhancing the cleavage rate of precursor tRNA by RNase P RNAs (by 60fold), RPP21/RPP29 contributes to increased substrate affinity (by 16fold) | Methanothermobacter thermautotrophicus |
oligomer | the archaeal holoenzyme is associated with 1 RNase P RNA and at least 4 RNase P proteins (POP5, RPP30, RPP21 and RPP29). Archaeal RNase P proteins function as two binary RNase P protein complexes (POP5/RPP30 and RPP21/RPP29). Archaeal POP5/RPP30 reconstituted with bacterial and organellar RNase P RNAs. While POP5/RPP30 is solely responsible for enhancing the cleavage rate of precursor tRNA by RNase P RNAs (by 60fold), RPP21/RPP29 contributes to increased substrate affinity (by 16fold) | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
RNase P | - |
Methanothermobacter thermautotrophicus |
RNase P | - |
Pyrococcus furiosus |
RNase P | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Methanothermobacter thermautotrophicus |
55 | - |
assay at | Pyrococcus furiosus |
55 | - |
assay at | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Methanothermobacter thermautotrophicus |
7.5 | - |
assay at | Pyrococcus furiosus |
7.5 | - |
assay at | Methanocaldococcus jannaschii |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme catalyzes the Mg2+-dependent 5'-maturation of precursor tRNAs | Methanothermobacter thermautotrophicus |
physiological function | the enzyme catalyzes the Mg2+-dependent 5'-maturation of precursor tRNAs | Pyrococcus furiosus |
physiological function | the enzyme catalyzes the Mg2+-dependent 5'-maturation of precursor tRNAs | Methanocaldococcus jannaschii |