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Literature summary for 3.1.26.5 extracted from

  • Cho, I.M.; Lai, L.B.; Susanti, D.; Mukhopadhyay, B.; Gopalan, V.
    Ribosomal protein L7Ae is a subunit of archaeal RNase P (2010), Proc. Natl. Acad. Sci. USA, 107, 14573-14578.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information ribosomal protein L7Ae is a subunit of archaeal RNase P. Addition of L7Ae to this RNase P complex increases the optimal reaction temperature and kcat/Km (by approximately 360fold) for pre-tRNA cleavage to those observed with partially purified native enzyme Methanococcus maripaludis

Protein Variants

Protein Variants Comment Organism
N32A/E33A/K36A mutant protein L7Ae displays only 4% of the activity observed with the wild-type protein L7Ae Methanococcus maripaludis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000044
-
pre-tRNATyr 37°C, pH 7.5, in the presence of L7Ae Methanococcus maripaludis
0.0026
-
pre-tRNATyr 37°C, pH 7.5, in the absence of L7Ae Methanococcus maripaludis

Organism

Organism UniProt Comment Textmining
Methanococcus maripaludis P62426 and P60780 and P60781 and P60832 and P62378 P62426: ribosomal protein L7Ae, P60780: subunit Pop5, P60781: subunit rnp3, P60832: subunit rnp1, P62378: subunit rnp4
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-tRNATyr + H2O
-
Methanococcus maripaludis tRNATyr + 5'-oligoribonucleotide
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
36 38 optimal assay temperature of in vitro reconstituted enzyme in the absence of ribosomal protein L7Ae Methanococcus maripaludis
48 50 optimal assay temperature of in vitro reconstituted enzyme in the presence of ribosomal protein L7Ae Methanococcus maripaludis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
ribosomal protein L7Ae increases the thermostability of the RNase P holoenzyme, the secondary structure is not different from that of the wild type enzyme Methanococcus maripaludis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.17
-
pre-tRNATyr 37°C, pH 7.5, in the absence of L7Ae Methanococcus maripaludis
1.05
-
pre-tRNATyr 37°C, pH 7.5, in the presence of L7Ae Methanococcus maripaludis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
65.4
-
pre-tRNATyr 37°C, pH 7.5, in the absence of L7Ae Methanococcus maripaludis
23860
-
pre-tRNATyr 37°C, pH 7.5, in the presence of L7Ae Methanococcus maripaludis