Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | ribosomal protein L7Ae is a subunit of archaeal RNase P. Addition of L7Ae to this RNase P complex increases the optimal reaction temperature and kcat/Km (by approximately 360fold) for pre-tRNA cleavage to those observed with partially purified native enzyme | Methanococcus maripaludis |
Protein Variants | Comment | Organism |
---|---|---|
N32A/E33A/K36A | mutant protein L7Ae displays only 4% of the activity observed with the wild-type protein L7Ae | Methanococcus maripaludis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000044 | - |
pre-tRNATyr | 37°C, pH 7.5, in the presence of L7Ae | Methanococcus maripaludis | |
0.0026 | - |
pre-tRNATyr | 37°C, pH 7.5, in the absence of L7Ae | Methanococcus maripaludis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanococcus maripaludis | P62426 and P60780 and P60781 and P60832 and P62378 | P62426: ribosomal protein L7Ae, P60780: subunit Pop5, P60781: subunit rnp3, P60832: subunit rnp1, P62378: subunit rnp4 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNATyr + H2O | - |
Methanococcus maripaludis | tRNATyr + 5'-oligoribonucleotide | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
36 | 38 | optimal assay temperature of in vitro reconstituted enzyme in the absence of ribosomal protein L7Ae | Methanococcus maripaludis |
48 | 50 | optimal assay temperature of in vitro reconstituted enzyme in the presence of ribosomal protein L7Ae | Methanococcus maripaludis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
ribosomal protein L7Ae increases the thermostability of the RNase P holoenzyme, the secondary structure is not different from that of the wild type enzyme | Methanococcus maripaludis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
pre-tRNATyr | 37°C, pH 7.5, in the absence of L7Ae | Methanococcus maripaludis | |
1.05 | - |
pre-tRNATyr | 37°C, pH 7.5, in the presence of L7Ae | Methanococcus maripaludis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
65.4 | - |
pre-tRNATyr | 37°C, pH 7.5, in the absence of L7Ae | Methanococcus maripaludis | |
23860 | - |
pre-tRNATyr | 37°C, pH 7.5, in the presence of L7Ae | Methanococcus maripaludis |