Literature summary for 3.1.26.5 extracted from

Perederina, A.; Esakova, O.; Quan, C.; Khanova, E.; Krasilnikov, A.S.
Crystallization and preliminary X-ray diffraction analysis of the P3 RNA domain of yeast ribonuclease MRP in a complex with RNase P/MRP protein components Pop6 and Pop7 (2010), Acta Crystallogr. Sect. F, 66, 76-80.

Cloned(Commentary)

Cloned (Comment)	Organism
full-length Pop6 and Pop7 coexpressed in Escherichia coli. Selenomethionine derivative of the Pop6-Pop7 heterodimer obtained using Escherichia coli strain BL21	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
complex including a circularly permuted 46-nucleotide-long P3 domain of the RNA component of ribonuclease MRP and selenomethionine derivatives of the shared ribonuclease P/MRP protein components Pop6 and Pop7, using the sitting-drop vapour-diffusion method. The crystals belong to space group P4222 with unit-cell parameters a = b = 127.2, c = 76.8 A , alpha = beta = gamma = 90° and diffract to 3.25 A resolution. The terminal part of the proximal helical stem of the P3 domain is not involved in interactions with Pop6-Pop7	Saccharomyces cerevisiae

Crystallization (Comment)

Organism

complex including a circularly permuted 46-nucleotide-long P3 domain of the RNA component of ribonuclease MRP and selenomethionine derivatives of the shared ribonuclease P/MRP protein components Pop6 and Pop7, using the sitting-drop vapour-diffusion method. The crystals belong to space group P4222 with unit-cell parameters a = b = 127.2, c = 76.8 A , alpha = beta = gamma = 90° and diffract to 3.25 A resolution. The terminal part of the proximal helical stem of the P3 domain is not involved in interactions with Pop6-Pop7

Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for formation of the Pop6-Pop7-RNA complex	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Pop6 and Pop7	Saccharomyces cerevisiae

Storage Stability

Storage Stability	Organism
4°C, 10 mM Tris-HCl buffer, pH 7.4, 100 mM NaCl, 50 mM KCl, 2 mM DTT, 0.1 mM Na EDTA and 0.1 mM PMSF, several weeks	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
Pop6	-	Saccharomyces cerevisiae
Pop7	-	Saccharomyces cerevisiae
ribonuclease MRP	-	Saccharomyces cerevisiae
ribonuclease P	-	Saccharomyces cerevisiae
RNase P	-	Saccharomyces cerevisiae
RNase P/MRP protein	-	Saccharomyces cerevisiae