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Literature summary for 3.1.26.5 extracted from

  • Kazantsev, A.V.; Krivenko, A.A.; Pace, N.R.
    Mapping metal-binding sites in the catalytic domain of bacterial RNase P RNA (2009), RNA, 15, 266-276.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.5

Crystallization (Commentary)

Crystallization (Comment) Organism
the P7DELTA RNA which is comprised of the independently folding catalytic domain of the RNase P RNA is crystallized by vapour diffusion method with 50 mM Na-cacodylate at pH 6.5, 100 mM KCL, 0.7-1.0 mM spermidine tetrachloride, and 21-23% 1,6-hexanediol Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for folding, substrate binding, and catalysis Geobacillus stearothermophilus
additional information Mn2+, Co2+, Ni2+, Cu2+, Au3+, Pb2+, La3+, Pr3+, Sm3+, Gd3+, Dy3+, Yb3+, and Lu3+ are able to bind to RNase P but are not specific proxies for Mg2+ Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q sepharose column chromatography Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-tRNA + H2O
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 Geobacillus stearothermophilus tRNA + 5'-oligoribonucleotide
-
 ?
pre-tRNA precursor + H2O
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 Geobacillus stearothermophilus tRNA + 5'-oligoribonucleotide
-
 ?

Synonyms

Synonyms Comment Organism
RNase P
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 Geobacillus stearothermophilus