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Literature summary for 3.1.26.5 extracted from

  • Vourekas, A.; Vryzaki, E.; Toumpeki, C.; Stamatopoulou, V.; Monastirli, A.; Tsambaos, D.; Drainas, D.
    Partial purification and characterization of RNase P from human peripheral lymphocytes (2009), Exp. Dermatol., 18, 130-133.
    View publication on PubMed
Search for more literature for 3.1.26.5

Inhibitors

Inhibitors Comment Organism Structure
neomycin B the inhibition is of non-competitive type, increasing Mg2+ concentrations from 3 to 20 mM, at a constant neomycin concentration (0.05 mM), result in a considerable recovery of the activity (about 40%) Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000244
-
 SupS1 precursor in 50 mM Tris /HCl pH 7.5, 100 mM NH4Cl, 5 mM MgCl2, 2.5 mM EGTA and 0.5 U Rnasin Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on, the optimum is at 5 mM MgCl2, when reactions are carried out at pH 7.5 and 37°C Homo sapiens
NH4+ dependent on, the optimum is at 70 mM NH4Cl, when reactions are carried out at pH 7.5 and 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
phosphocellulose column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
lymphocyte
-
 Homo sapiens
-

Storage Stability

Storage Stability Organism
-70°C, 50 mM Tris /HCl pH 7.5, 100 mM NH4Cl, 5 mm MgCl2, 2.5 mM EGTA, and 0.5 U RNasin, few weeks, the enzyme activity remains stable Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-tRNA + H2O RNase P is a key enzyme acting early in the tRNA biogenesis pathway, which catalyses the endonucleolytic cleavage of the 5' leader sequence of precursor tRNAs and generates their 5' mature end Homo sapiens tRNA + 5'-oligoribonucleotide
-
 ?
SupS1 precursor + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
RNase P
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.037
-
 neomycin B in 50 mM Tris /HCl pH 7.5, 100 mM NH4Cl, 5 mM MgCl2, 2.5 mM EGTA and 0.5 U Rnasin Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.074
-
 in 50 mM Tris /HCl pH 7.5, 100 mM NH4Cl, 5 mM MgCl2, 2.5 mM EGTA and 0.5 U Rnasin Homo sapiens neomycin B