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Literature summary for 3.1.26.5 extracted from
- Characterization of ribonuclease P from the archaebacterium Sulfolobus solfataricus. (1990), J. Biol. Chem., 265, 12927-12932.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| spermine | A 2-fold increase in enzyme activity is observed when 1.25 mM spermine is included in the reaction mixture. | Saccharolobus solfataricus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | inhibited at concentrations greater than 7.5 mM | Saccharolobus solfataricus | |
| NH4+ | inhibited at ammonium concentrations greater than 100 mM in the presence of 7.5 mM MgC12 | Saccharolobus solfataricus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00025 | - |
tRNA | pH 7.5, 77°C | Saccharolobus solfataricus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimally at 7.5 mM | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 400000 | - |
gel exclusion chromatography | Saccharolobus solfataricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| precursor tRNA + H2O | Saccharolobus solfataricus | ribonuclease P is the endonuclease that removes the leader fragments from the 5'-ends of precursor tRNAs | 77- and 35-RNA fragments | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| RNase P from Sulfolubus solfataricus is purified 800fold by Trisacryl-DEAE, and Sepharose CL-6B chromatographies and Cs2S04 buoyant density centrifugation | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| precursor tRNA + H2O | ribonuclease P is the endonuclease that removes the leader fragments from the 5'-ends of precursor tRNAs | Saccharolobus solfataricus | 77- and 35-RNA fragments | - |
? | |
| tRNA + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribonuclease P | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 77 | - |
The temperature optimum for the reaction is 77 °C, reflecting the thermophilic character of the organism | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Saccharolobus solfataricus |
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