Literature summary for 3.1.26.5 extracted from

Thomas, B.C.; Chamberlain, J.; Engelke, D.R.; Gegenheimer, P.
Evidence for an RNA-based catalytic mechanism in eukaryotic nuclear ribonuclease P (2000), RNA, 6, 554-562.

Search for more literature for 3.1.26.5

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a pre-tRNA substrate in which the pro-Rp nonbridging oxygen of the scissile bond is replaced by sulfur	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
phosphorothionate	inhibition of activity due to phosphothionate substitution at the pro-Rp or pro-Sp nonbridging position of the scissile bond in tRNA, activity cannot be recovered by Mn2+	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleus	-	Saccharomyces cerevisiae	5634	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent on, binds to the pro-Rp nonbridging oxygen of the scissile bond, coordination	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
tRNA precursor + H2O	Saccharomyces cerevisiae	enzyme is responsible for removing the 5'-leader segment of precursor tRNA	mature tRNA + 5'-terminal oligonucleotide	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
ribonucleoprotein	-	Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment)	Organism
partially	Saccharomyces cerevisiae

Reaction

Reaction	Comment	Organism	Reaction ID
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor	an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, RNA-based catalytic mechanism	Saccharomyces cerevisiae	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	no activity with substrates in which the pro-Rp or pro-Sp nonbridging oxygen of the scissile bond is replaced by sulfur, substitution via phosphothionate	Saccharomyces cerevisiae	?	-	?
tRNA precursor + H2O	enzyme is responsible for removing the 5'-leader segment of precursor tRNA	Saccharomyces cerevisiae	mature tRNA + 5'-terminal oligonucleotide	-	?
tRNAPhe (A+1) precursor + H2O	yeast tRNA substrate from in vitro transcription, structure	Saccharomyces cerevisiae	mature tRNAPhe + 5'-terminal oligonucleotide	5'-terminal oligonucleotide with 3'-hydroxyl and 5'-phosphoryl, product and cleavage site determination	?
tRNAPhe (G+1) precursor + H2O	yeast tRNA substrate from in vitro transcription, structure	Saccharomyces cerevisiae	mature tRNAPhe + 5'-terminal oligonucleotide	5'-terminal oligonucleotide with 3'-hydroxyl and 5'-phosphoryl, product and cleavage site determination	?

Subunits

Subunits	Comment	Organism
More	enzyme is a ribonucleoprotein consisting of multiple protein components and a single RNA species	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
RNase P	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)