Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by phosphothionate | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000012 | - |
tRNAPhe (G+1) precursor | 37°C, substrate substituted with phosphothionate from GMPaS | Spinacia oleracea | |
0.000016 | - |
tRNAPhe (G+1) precursor | 37°C | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mg2+ is not required for activity | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no ribonucleoprotein | - |
Spinacia oleracea |
Purification (Comment) | Organism |
---|---|
partially from 800-900fold over the chloroplast lysate | Spinacia oleracea |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, chloroplast enzyme does not use the ribozyme-type pre-tRNA cleavage mechanism, distinct from bacterial reaction mechanism | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNAPhe (A+1) precursor + H2O | yeast tRNA substrate from in vitro transcription, structure, cleavage of substrate containing a pro-Rp nonbridging oxygen or, by substitution with phosphothionate, a sulfur at the scissile bond | Spinacia oleracea | mature tRNAPhe + 5'-terminal oligonucleotide | 5'-terminal oligonucleotide with 3'-hydroxyl and 5'-phosphoryl, product and cleavage site determination | ? | |
tRNAPhe (G+1) precursor + H2O | yeast tRNA substrate from in vitro transcription, structure, cleavage of substrate containing a pro-Rp nonbridging oxygen or, by substitution with phosphothionate, a sulfur at the scissile bond | Spinacia oleracea | mature tRNAPhe + 5'-terminal oligonucleotide | 5'-terminal oligonucleotide with 3'-hydroxyl and 5'-phosphoryl, product and cleavage site determination | ? | |
tRNAPhe precursor + H2O | maize tRNA substrate, structure | Spinacia oleracea | mature tRNAPhe + 5'-terminal oligonucleotide | cleavage site determination | ? |
Synonyms | Comment | Organism |
---|---|---|
RNase P | - |
Spinacia oleracea |
transfer RNA 5' maturation enzyme | - |
Spinacia oleracea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Spinacia oleracea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
tRNAPhe (G+1) precursor | 37°C, substrate substituted with phosphothionate from GMPaS | Spinacia oleracea | |
0.003 | - |
tRNAPhe (G+1) precursor | 37°C | Spinacia oleracea |