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Literature summary for 3.1.26.5 extracted from

  • Thomas, B.C.; Li, X.; Gegenheimer, P.
    Chloroplast ribonuclease P does not utilize the ribozyme-type pre-tRNA cleavage mechanism (2000), RNA, 6, 545-553.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by phosphothionate Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000012
-
tRNAPhe (G+1) precursor 37°C, substrate substituted with phosphothionate from GMPaS Spinacia oleracea
0.000016
-
tRNAPhe (G+1) precursor 37°C Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Spinacia oleracea 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+ is not required for activity Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no ribonucleoprotein
-
Spinacia oleracea

Purification (Commentary)

Purification (Comment) Organism
partially from 800-900fold over the chloroplast lysate Spinacia oleracea

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, chloroplast enzyme does not use the ribozyme-type pre-tRNA cleavage mechanism, distinct from bacterial reaction mechanism Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNAPhe (A+1) precursor + H2O yeast tRNA substrate from in vitro transcription, structure, cleavage of substrate containing a pro-Rp nonbridging oxygen or, by substitution with phosphothionate, a sulfur at the scissile bond Spinacia oleracea mature tRNAPhe + 5'-terminal oligonucleotide 5'-terminal oligonucleotide with 3'-hydroxyl and 5'-phosphoryl, product and cleavage site determination ?
tRNAPhe (G+1) precursor + H2O yeast tRNA substrate from in vitro transcription, structure, cleavage of substrate containing a pro-Rp nonbridging oxygen or, by substitution with phosphothionate, a sulfur at the scissile bond Spinacia oleracea mature tRNAPhe + 5'-terminal oligonucleotide 5'-terminal oligonucleotide with 3'-hydroxyl and 5'-phosphoryl, product and cleavage site determination ?
tRNAPhe precursor + H2O maize tRNA substrate, structure Spinacia oleracea mature tRNAPhe + 5'-terminal oligonucleotide cleavage site determination ?

Synonyms

Synonyms Comment Organism
RNase P
-
Spinacia oleracea
transfer RNA 5' maturation enzyme
-
Spinacia oleracea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Spinacia oleracea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0005
-
tRNAPhe (G+1) precursor 37°C, substrate substituted with phosphothionate from GMPaS Spinacia oleracea
0.003
-
tRNAPhe (G+1) precursor 37°C Spinacia oleracea