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Literature summary for 3.1.26.5 extracted from

  • Hansen, A.; Pfeiffer, T.; Zuleeg, T.; Limmer, S.; Ciesiolka, J.; Feltens, R.; Hartmann, R.K.
    Exploring the minimal substrate requirements for trans-cleavage by RNase P holoenzymes from Escherichia coli and Bacillus subtilis (2001), Mol. Microbiol., 41, 131-143.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type enzyme by in vitro transcription, expression of His-tagged enzyme Bacillus subtilis
expression of wild-type enzyme by in vitro transcription, expression of His-tagged enzyme Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information construction of mutant enzyme chimeras consisting of either Escherichia coli protein components and Bacillus subtilis RNA component or vice versa Bacillus subtilis
additional information construction of mutant enzyme chimeras consisting of either Escherichia coli protein components and Bacillus subtilis RNA component or vice versa Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Bacillus subtilis
additional information
-
additional information kinetics Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pre-tRNA + H2O Bacillus subtilis
-
mature tRNA + 5'-terminal oligonucleotide
-
?
pre-tRNA + H2O Escherichia coli
-
mature tRNA + 5'-terminal oligonucleotide
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant untagged and His-tagged enzymes Bacillus subtilis
recombinant untagged and His-tagged enzymes Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, cleavage of ssRNA, mechanism Bacillus subtilis
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, cleavage of ssRNA, mechanism Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate requirements for trans-cleavage of wild-type and mutant hybrid enzymes, overview Bacillus subtilis ?
-
?
additional information substrate requirements for trans-cleavage of wild-type and mutant hybrid enzymes, overview Escherichia coli ?
-
?
pre-tRNA + H2O
-
Bacillus subtilis mature tRNA + 5'-terminal oligonucleotide
-
?
pre-tRNA + H2O
-
Escherichia coli mature tRNA + 5'-terminal oligonucleotide
-
?
pre-tRNA mimic + H2O small bipartite model substrate, smallest mimic of natural enzyme substrate consisting of a 4 bp stem and a 1 nucleotide 5'-flank, specific trans-cleavage at the canonical enzyme cleavage site Escherichia coli ?
-
?
pre-tRNA mimic + H2O small bipartite model substrates with different numbering of stem and 5'-flank base pairs, specific trans-cleavage at the canonical enzyme cleavage site, overview Bacillus subtilis ?
-
?
pre-tRNA mimic a + H2O mimic of natural enzyme substrate consisting of a 7 bp stem and a 3 nucleotide 5'-flank Bacillus subtilis ?
-
?
pre-tRNA mimic a + H2O mimic of natural enzyme substrate consisting of a 7 bp stem and a 3 nucleotide 5'-flank Escherichia coli ?
-
?
pre-tRNA mimic b + H2O mimic of natural enzyme substrate consisting of a 7 bp stem and a 1 nucleotide 5'-flank Bacillus subtilis ?
-
?
pre-tRNA mimic b + H2O mimic of natural enzyme substrate consisting of a 7 bp stem and a 1 nucleotide 5'-flank Escherichia coli ?
-
?
pre-tRNA mimic c + H2O mimic of natural enzyme substrate consisting of a 4 bp stem and a 3 nucleotide 5'-flank Bacillus subtilis ?
-
?
pre-tRNA mimic c + H2O mimic of natural enzyme substrate consisting of a 4 bp stem and a 3 nucleotide 5'-flank Escherichia coli ?
-
?
pre-tRNA mimic d + H2O mimic of natural enzyme substrate consisting of a 4 bp stem and a 1 nucleotide 5'-flank Bacillus subtilis ?
-
?
pre-tRNA mimic d + H2O mimic of natural enzyme substrate consisting of a 4 bp stem and a 1 nucleotide 5'-flank Escherichia coli ?
-
?
ssRNA oligonucleotide + H2O holoenzyme, specificity with different ssRNA substrates, determination of cleavage sites, overview Bacillus subtilis 5'-phospho-3'-hydroxy-ribonucleotides
-
?
ssRNA oligonucleotide + H2O holoenzyme, specificity with different ssRNA substrates, determination of cleavage sites, overview Escherichia coli 5'-phospho-3'-hydroxy-ribonucleotides
-
?

Synonyms

Synonyms Comment Organism
RNase P
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacillus subtilis
37
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Bacillus subtilis
additional information
-
additional information
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Bacillus subtilis
7
-
assay at Escherichia coli