Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutants inactive with pre-4.5S RNA but not with pre-tRNA | Bacteria |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inactivation | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | both pre-steady state and steady state kinetics | Bacteria | |
additional information | - |
additional information | pre-steady state kinetics, nuclear enzyme form | Saccharomyces cerevisiae | |
0.000055 | - |
tRNA precursor | nuclear enzyme form | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | enzyme form might be RNA-independent | Spinacia oleracea | 9507 | - |
mitochondrion | mitochondrial enzyme form of RNase MRP | Homo sapiens | 5739 | - |
mitochondrion | mitochondrial ribozyme form | Saccharomyces cerevisiae | 5739 | - |
additional information | subcellular localization of different enzyme forms | Homo sapiens | - |
- |
additional information | subcellular localization of different enzyme forms | Saccharomyces cerevisiae | - |
- |
nucleolus | nucleolar enzyme form of RNase MRP | Homo sapiens | 5730 | - |
nucleus | nuclear ribozyme form RNase P in nucleoplasm | Homo sapiens | 5634 | - |
nucleus | nuclear ribozyme forms MPR and P | Saccharomyces cerevisiae | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | high activation | Bacteria | |
Mg2+ | high activation | Bacillus subtilis | |
Mg2+ | high activation | Homo sapiens | |
Mg2+ | high activation, specific for | Saccharomyces cerevisiae | |
Mn2+ | can substitute for Mg2+, slightly lower activity | Bacteria | |
Mn2+ | can substitute for Mg2+, slightly lower activity | Bacillus subtilis | |
Mn2+ | can substitute for Mg2+, slightly lower activity | Homo sapiens | |
Mn2+ | can substitute for Mg2+, slightly lower activity | Saccharomyces cerevisiae | |
additional information | divalent metal ions are absolutely required, reduced activity with Ca2+ | Bacteria | |
additional information | divalent metal ions are absolutely required, reduced activity with Ca2+ | Bacillus subtilis | |
additional information | divalent metal ions are absolutely required, reduced activity with Ca2+ | Homo sapiens | |
additional information | divalent metal ions are absolutely required, reduced activity with Ca2+ | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15500 | - |
1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 | Saccharomyces cerevisiae |
15800 | - |
1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 | Saccharomyces cerevisiae |
16300 | - |
1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 | Saccharomyces cerevisiae |
18200 | - |
1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 | Saccharomyces cerevisiae |
19600 | - |
1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 | Saccharomyces cerevisiae |
22500 | - |
1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 | Saccharomyces cerevisiae |
22600 | - |
1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 | Saccharomyces cerevisiae |
32200 | - |
1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 | Saccharomyces cerevisiae |
32900 | - |
1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 | Saccharomyces cerevisiae |
100500 | - |
1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 | Saccharomyces cerevisiae |
112000 | - |
1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 | Saccharomyces cerevisiae |
120000 | - |
1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10Sa RNA + H2O | Bacteria | - |
? | - |
? | |
4.55 rRNA precursor + H2O | Bacteria | - |
mature 4.55 rRNA + 5'-oligonucleotide | - |
? | |
additional information | Saccharomyces cerevisiae | the mitochondrial ribozyme RNA subunit might have a cellular function outside the mitochondria | ? | - |
? | |
polycistronic his operon mRNA precursor + H2O | Bacteria | - |
? | - |
? | |
tRNA precursor + H2O | Cyanophora paradoxa | cleavage of 5'-terminal oligonucleotide, maturation | mature tRNA + 5'-oligonucleotide | - |
? | |
tRNA precursor + H2O | Bacteria | cleavage of 5'-terminal oligonucleotide, maturation | mature tRNA + 5'-oligonucleotide | - |
? | |
tRNA precursor + H2O | Bacillus subtilis | cleavage of 5'-terminal oligonucleotide, maturation | mature tRNA + 5'-oligonucleotide | - |
? | |
tRNA precursor + H2O | Homo sapiens | cleavage of 5'-terminal oligonucleotide, maturation | mature tRNA + 5'-oligonucleotide | - |
? | |
tRNA precursor + H2O | Saccharomyces cerevisiae | cleavage of 5'-terminal oligonucleotide, maturation | mature tRNA + 5'-oligonucleotide | - |
? | |
tRNA precursor + H2O | Spinacia oleracea | cleavage of 5'-terminal oligonucleotide, maturation | mature tRNA + 5'-oligonucleotide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
enzyme is a catalytically active RNA with only a small additional protein subunit | - |
Bacteria | - |
enzyme is a catalytically active RNA, at least 2 isoforms with different substrate specificities | - |
Cyanophora paradoxa | - |
- |
- |
Homo sapiens | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Spinacia oleracea | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
ribonucleoprotein | enzyme is a ribonucleoprotein with a large protein part and 1 RNA subunit | Cyanophora paradoxa |
ribonucleoprotein | enzyme is a ribonucleoprotein with a large protein part and 1 RNA subunit | Homo sapiens |
ribonucleoprotein | enzyme is a ribonucleoprotein with a large protein part and 1 RNA subunit | Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
partially | Spinacia oleracea |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, non-RNA-based cleavage mechanism, structure-function relationship of eukaryotic enzymes | Spinacia oleracea | |
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, reaction mechanism in 3 consecutive steps: pre-tRNA binding, hydrolysis of the scissile phosphodiester bond, generating a 3'-hydroxyl and a 5'-phosphate group, possibly via a trigonal bipyramide, intermediate, and the dissociation of the mature tRNA and the 5'-leader product, structure-function relationship of eukaryotic enzymes | Homo sapiens | |
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, reaction mechanism in 3 consecutive steps: pre-tRNA binding, hydrolysis of the scissile phosphodiester bond, generating a 3'-hydroxyl and a 5'-phosphate group, possibly via a trigonal bipyramide, intermediate, and the dissociation of the mature tRNA and the 5'-leader product, substrate recognition mechanism, overview, structure-function realtionship of eukaryotic enzymes | Saccharomyces cerevisiae | |
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, substrate recognition, enzyme possesses a single-stranded RNA binding cleft that interacts with the unpaired 5'-leader | Bacillus subtilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
leaf | - |
Spinacia oleracea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10Sa RNA + H2O | - |
Bacteria | ? | - |
? | |
4.55 rRNA precursor + H2O | - |
Bacteria | mature 4.55 rRNA + 5'-oligonucleotide | - |
? | |
additional information | the mitochondrial ribozyme RNA subunit might have a cellular function outside the mitochondria | Saccharomyces cerevisiae | ? | - |
? | |
additional information | substrate specificities of enzyme forms RNase P and RNase MRP | Homo sapiens | ? | - |
? | |
additional information | substrate specificities of enzyme forms RNase P and RNase MRP | Saccharomyces cerevisiae | ? | - |
? | |
polycistronic his operon mRNA precursor + H2O | - |
Bacteria | ? | - |
? | |
tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation | Cyanophora paradoxa | mature tRNA + 5'-oligonucleotide | - |
? | |
tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation | Bacteria | mature tRNA + 5'-oligonucleotide | - |
? | |
tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation | Bacillus subtilis | mature tRNA + 5'-oligonucleotide | - |
? | |
tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation | Homo sapiens | mature tRNA + 5'-oligonucleotide | - |
? | |
tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation | Saccharomyces cerevisiae | mature tRNA + 5'-oligonucleotide | - |
? | |
tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation | Spinacia oleracea | mature tRNA + 5'-oligonucleotide | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 | Saccharomyces cerevisiae |
More | subunit composition for RNase P: 1 RNA subunit RPM1 plus at least 1 protein subunit Rpm2p for the mitochondrial ribozyme, 9 protein components for the nuclear enzyme form, namely Pop1p, Pop3p-Pop8p, Rpp1p, and Rpr2p, secondary structure of nuclear enzyme RNA, subunit composition for RNase MRP: 1 RNA subunit NME1 plus 9 protein components for the nuclear enzyme form, namely Pop1p, Pop3p-Pop7p, Pop, Rpp1p, and SNM1, overview, Pop7p is also known as Rpp2p | Saccharomyces cerevisiae |
oligomer | subunit composition, 1 RNA subunit, i.e. H1 for RNase P or 7-2 for RNase MRP, plus 9 protein components namely Pop1p, Rpp29p, Rpp20p, Rpp30p, Rep38p, Rpp40p, Rpp25p, and Rpp14p for RNase P, or pls 4 protein components namely Pop1p, Rpp29p, Rpp20p, and Rpp30p for RNase MRP, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
RNase MRP | - |
Homo sapiens |
RNase MRP | - |
Saccharomyces cerevisiae |
RNase P | - |
Cyanophora paradoxa |
RNase P | - |
Bacteria |
RNase P | - |
Bacillus subtilis |
RNase P | - |
Homo sapiens |
RNase P | - |
Saccharomyces cerevisiae |
RNase P | - |
Spinacia oleracea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Bacteria | |
1.3 | - |
tRNA precursor | nuclear enzyme form | Saccharomyces cerevisiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharomyces cerevisiae | protein subunits Pop1p, Pop3p, Pop4p, Pop6p, Pop7p, Rpp1p, and Rpr2p | 4.7 | 4.6 |
Saccharomyces cerevisiae | protein subunit Pop8p | - |
4.9 |
Saccharomyces cerevisiae | protein subunit Pop5p | - |
6.9 |