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Literature summary for 3.1.26.5 extracted from

  • Harris, M.E.; Christian, E.L.
    Recent insights into the structure and function of the ribonucleoprotein enzyme ribonuclease P (2003), Curr. Opin. Struct. Biol., 13, 325-333.
    View publication on PubMed
Search for more literature for 3.1.26.5

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallographic structure of the S-domain of the enzyme RNA subunit Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ changes the cleavage pattern Bacillus subtilis
Cd2+ changes the cleavage pattern Escherichia coli
Cu2+ changes the cleavage pattern Bacillus subtilis
Cu2+ changes the cleavage pattern Escherichia coli
Mg2+ interaction with the helix P4 Bacillus subtilis
Mg2+ interaction with the helix P4 Escherichia coli
Mn2+ substitution of Mg2+ by Mn2+ can result in miscleavage of the substrate containing a N(-1)/N(73) pair, better sulfur coordination compared to Mg2+ Bacillus subtilis
Mn2+ substitution of Mg2+ by Mn2+ can result in miscleavage of the substrate containing a N(-1)/N(73) pair, better sulfur coordination compared to Mg2+ Escherichia coli
additional information divalent metal cations are essential for catalysis and stabilize the enzyme conformation and subunit interaction Bacillus subtilis
additional information divalent metal cations are essential for catalysis and stabilize the enzyme conformation and subunit interaction Escherichia coli
Ni2+ changes the cleavage pattern Bacillus subtilis
Ni2+ changes the cleavage pattern Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus subtilis other pre-RNAs are also physiological substrates of the enzyme ?
-
 ?
additional information Escherichia coli other pre-RNAs are also physiological substrates of the enzyme ?
-
 ?
tRNA precursor + H2O Bacillus subtilis tRNA processing mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O Escherichia coli tRNA processing mature tRNA + 5'-oligonucleotide
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Escherichia coli
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
ribonucleoprotein
-
 Bacillus subtilis
ribonucleoprotein
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, the protein subunit is essential for substrate binding, the RNA subunit is essential for catalysis Bacillus subtilis
a
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, the protein subunit is essential for substrate binding, the RNA subunit is essential for catalysis Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information other pre-RNAs are also physiological substrates of the enzyme Bacillus subtilis ?
-
 ?
additional information other pre-RNAs are also physiological substrates of the enzyme Escherichia coli ?
-
 ?
additional information structural basis and mechanism of substrate specificity, global structure of the enzyme-substrate complex Bacillus subtilis ?
-
 ?
additional information structural basis and mechanism of substrate specificity, global structure of the enzyme-substrate complex Escherichia coli ?
-
 ?
tRNA precursor + H2O tRNA processing Bacillus subtilis mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O tRNA processing Escherichia coli mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide, cleavage site Bacillus subtilis mature tRNA + 5'-oligonucleotide generates 5'-phosphate,3'-hydroxyl-product ?
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide, cleavage site Escherichia coli mature tRNA + 5'-oligonucleotide generates 5'-phosphate,3'-hydroxyl-product ?

Subunits

Subunits Comment Organism
More enzyme is composed of a large RNA subunit of about 400 nucleotides, and a small protein subunit of about 100 amino acids, global structure of the enzyme-substrate complex Bacillus subtilis
More enzyme is composed of a large RNA subunit of about 400 nucleotides, and a small protein subunit of about 100 amino acids, global structure of the enzyme-substrate complex, secondary structure of the enzyme RNA subunit Escherichia coli

Synonyms

Synonyms Comment Organism
RNase P
-
 Bacillus subtilis
RNase P
-
 Escherichia coli
tRNA-processing endonuclease
-
 Bacillus subtilis
tRNA-processing endonuclease
-
 Escherichia coli