Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guide DNA | complete inhibition at 0.005 mM | Bacillus subtilis | |
guide DNA | complete inhibition at 0.005 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000072 | - |
human pre-tRNATyr | pH 8.0, 37°C, 20 mM Mg2+ | Bacillus subtilis | |
0.00025 | - |
human pre-tRNATyr | pH 8.0, 37°C, 40 mM Mg2+ | Bacillus subtilis | |
0.00043 | - |
human pre-tRNATyr | pH 8.0, 37°C, 20 mM Mg2+ | Escherichia coli | |
0.00054 | - |
human pre-tRNATyr | pH 8.0, 37°C, 40 mM Mg2+ | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolutely required, optimal at about 10 mM, for catalysis and substrate shape recognition, influences substrate binding affinity | Escherichia coli | |
Mg2+ | absolutely required, optimal at about 20 mM, for catalysis and substrate shape recognition, influences substrate binding affinity | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNA precursor + H2O | Bacillus subtilis | tRNA processing | mature tRNA + 5'-oligonucleotide | - |
? | |
tRNA precursor + H2O | Escherichia coli | tRNA processing | mature tRNA + 5'-oligonucleotide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Escherichia coli | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
ribonucleoprotein | - |
Bacillus subtilis |
ribonucleoprotein | - |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
human pre-tRNATyr + H2O | - |
Bacillus subtilis | human mature tRNATyr + 5'-oligonucleotide | - |
? | |
human pre-tRNATyr + H2O | - |
Escherichia coli | human mature tRNATyr + 5'-oligonucleotide | - |
? | |
tRNA precursor + H2O | tRNA processing | Bacillus subtilis | mature tRNA + 5'-oligonucleotide | - |
? | |
tRNA precursor + H2O | tRNA processing | Escherichia coli | mature tRNA + 5'-oligonucleotide | - |
? | |
tRNATyr precursor + H2O | human substrate, cleavage of 5'-terminal oligonucleotide, enzyme recognizes the RNA substrate hairpin structure | Bacillus subtilis | mature tRNATyr + 5'-oligonucleotide | generates 5'-phosphate,3'-hydroxyl-product | ? | |
tRNATyr precursor + H2O | human substrate, cleavage of 5'-terminal oligonucleotide, enzyme recognizes the RNA substrate hairpin structure | Escherichia coli | mature tRNATyr + 5'-oligonucleotide | generates 5'-phosphate,3'-hydroxyl-product | ? |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease P ribozyme | - |
Bacillus subtilis |
ribonuclease P ribozyme | - |
Escherichia coli |
RNase P | - |
Bacillus subtilis |
RNase P | - |
Escherichia coli |
RNase P ribozyme | - |
Bacillus subtilis |
RNase P ribozyme | - |
Escherichia coli |
transfer RNA processing enzyme | - |
Bacillus subtilis |
transfer RNA processing enzyme | - |
Escherichia coli |
tRNA-processing enzyme | - |
Bacillus subtilis |
tRNA-processing enzyme | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000011 | - |
human pre-tRNATyr | pH 8.0, 37°C, 20 mM Mg2+ | Bacillus subtilis | |
0.00002 | - |
human pre-tRNATyr | pH 8.0, 37°C, 20 mM Mg2+ | Escherichia coli | |
0.00004 | - |
human pre-tRNATyr | pH 8.0, 37°C, 40 mM Mg2+ | Escherichia coli | |
0.000056 | - |
human pre-tRNATyr | pH 8.0, 37°C, 40 mM Mg2+ | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bacillus subtilis |
8 | - |
assay at | Escherichia coli |