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Literature summary for 3.1.26.5 extracted from
- Characterization of RNase P holoenzymes from Methanococcus jannaschii and Methanothermobacter thermoautotrophicus (2001), Biol. Chem., 382, 1171-1177.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | irreversible inactivation | Methanocaldococcus jannaschii |  |
| Ca2+ | irreversible inactivation | Methanothermobacter thermautotrophicus | |
| EDTA | irreversible inactivation | Methanocaldococcus jannaschii | |
| EDTA | irreversible inactivation | Methanothermobacter thermautotrophicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000345 | - |
tRNA precursor | pH 8.0, 65°C, in presence of 800 mM ammonium acetate and 5 mM MgCl2 | Methanocaldococcus jannaschii | |
| 0.0000684 | - |
tRNA precursor | pH 8.0, 50°C, in presence of 50 mM ammonium acetate and 30 mM MgCl2 | Methanothermobacter thermautotrophicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on, at least half-maximal activity between 8-80 mM | Methanothermobacter thermautotrophicus | |
| Mg2+ | dependent on, optimal at 2-10 mM | Methanocaldococcus jannaschii | |
| additional information | at least half-maximal activity at 1 M ammonium acetate | Methanothermobacter thermautotrophicus | |
| additional information | optimal ionic strength at 800 mM ammonium acetate at 60°C | Methanocaldococcus jannaschii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
about, gel filtration | Methanocaldococcus jannaschii |
| 400000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Methanothermobacter thermautotrophicus | phylogenetic study of archeal enzymes | ? | - |
? | |
| additional information | Methanocaldococcus jannaschii | phylogenetic study of archeal enzymes | ? | - |
? | |
| tRNA precursor + H2O | Methanothermobacter thermautotrophicus | cleavage of 5'-terminal oligonucleotide, maturation | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | Methanocaldococcus jannaschii | cleavage of 5'-terminal oligonucleotide, maturation | mature tRNA + 5'-oligonucleotide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
type M enzyme | - |
| Methanothermobacter thermautotrophicus | - |
formerly Methanobacterium thermoautotrophicum strain DELTAH, type A enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Methanothermobacter thermautotrophicus |
| partially, 2400fold | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | phylogenetic study of archeal enzymes | Methanothermobacter thermautotrophicus | ? | - |
? | |
| additional information | phylogenetic study of archeal enzymes | Methanocaldococcus jannaschii | ? | - |
? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation | Methanothermobacter thermautotrophicus | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation | Methanocaldococcus jannaschii | mature tRNA + 5'-oligonucleotide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | subunit composition, secondary structure of the enzyme RNA moiety | Methanothermobacter thermautotrophicus |
| More | subunit composition, secondary structure of the enzyme RNA moiety | Methanocaldococcus jannaschii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase P | - |
Methanothermobacter thermautotrophicus |
| RNase P | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
about | Methanothermobacter thermautotrophicus |
| 80 | - |
about | Methanocaldococcus jannaschii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 80 | and above, 50% activity at about 70°C | Methanocaldococcus jannaschii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.57 | - |
tRNA precursor | pH 8.0, 50°C, in presence of 50 mM ammonium acetate and 30 mM MgCl2 | Methanothermobacter thermautotrophicus | |
| 0.88 | - |
tRNA precursor | pH 8.0, 65°C, in presence of 800 mM ammonium acetate and 5 mM MgCl2 | Methanocaldococcus jannaschii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Methanothermobacter thermautotrophicus |
| 8 | - |
assay at | Methanocaldococcus jannaschii |
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