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Literature summary for 3.1.26.4 extracted from

  • Liang, X.H.; Sun, H.; Nichols, J.G.; Crooke, S.T.
    RNase H1-dependent antisense oligonucleotides are robustly active in directing RNA cleavage in both the cytoplasm and the nucleus (2017), Mol. Ther., 25, 2075-2092 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.4

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
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 Homo sapiens 5737
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mitochondrion in the cytoplasm, RNase H1 is enriched in the mitochondria Homo sapiens 5739
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additional information RNase H1 localizes in both the nucleus and cytoplasm Homo sapiens
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-
nucleus
-
 Homo sapiens 5634
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Organism

Organism UniProt Comment Textmining
Homo sapiens O60930
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
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 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information degradation of cleaved mRNA fragments by RNase H1-dependent antisense oligonucleotides (ASOs). Expression of a cytoplasm-localized mutant 7SL RNA that contains a partial U16 small nucleolar RNA (snoRNA) sequence and treatement with an RNase H1-dependent antisense oligonucleotide (ASO) simultaneously reduces both the nuclear U16 snoRNA and the cytoplasmic 7SL mutant RNA as early as 30 min after transfection in an RNase H1-dependent manner. Both the 5' and 3' cleavage products of the 7SL mutant RNA are accumulated in the cytoplasm. Some ASOs can rapidly reduce mature mRNAs without reducing pre-mRNA levels, overview Homo sapiens ?
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 ?

Synonyms

Synonyms Comment Organism
RNase H1
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 Homo sapiens

General Information

General Information Comment Organism
metabolism the degradation of cleaved mRNA fragments by RNase H1-dependent ASOs involves XRN1, a cytoplasm-localized exonuclease, whereas the degradation of cleavage fragments of nuclear RNAs largely depend on XRN2, a nuclear-localized exonuclease Homo sapiens
physiological function RNase H1-dependent antisense oligonucleotides (ASOs) can direct RNase H1 cleavage of target RNAs. ASOs are robustly active in directing RNA cleavage in both the cytoplasm and the nucleus. ASOs are effective in reducing the levels of targeted small nuclear RNAs (snRNAs), small cajal body RNAs (scaRNAs), small nucleolar RNAs (snoRNAs), and nucleoplasmic long non-coding RNAs (lncRNAs) and premRNAs. In the cytoplasm, RNase H1 is enriched in the mitochondria, where it is involved in mitochondrial DNA replication and RNA processing, by removing the RNA/DNA hybrids during replication and transcription. Pre-existing cytoplasmic mRNAs can be cleaved by RNase H1-ASO treatment. RNase H1-dependent ASOs reduce cytoplasmic mRNAs much faster than normal mRNA decay Homo sapiens