Literature summary for 3.1.26.4 extracted from

Davis, R.R.; Shaban, N.M.; Perrino, F.W.; Hollis, T.
Crystal structure of RNA-DNA duplex provides insight into conformational changes induced by RNase H binding (2015), Cell Cycle, 14, 668-673 .

Crystallization (Commentary)

Crystallization (Comment)	Organism
annealed RNA and DNA dodecanucleotides complexed with RNase H, synthetic constructs, sitting drop vapor diffusion method at room temperature in conditions containing 20% PEG 3350, 0.2 M magnesium formate, 10% 2-methyl-2,4-pentanediol, X-ray diffraction structure determination and analysis, molecular replacement using the structure of Bacillus halodurans RNA-DNA component of the RNase H complex (PDB ID 1ZBI) as the search model	Halalkalibacterium halodurans

Crystallization (Comment)

Organism

annealed RNA and DNA dodecanucleotides complexed with RNase H, synthetic constructs, sitting drop vapor diffusion method at room temperature in conditions containing 20% PEG 3350, 0.2 M magnesium formate, 10% 2-methyl-2,4-pentanediol, X-ray diffraction structure determination and analysis, molecular replacement using the structure of Bacillus halodurans RNA-DNA component of the RNase H complex (PDB ID 1ZBI) as the search model

Halalkalibacterium halodurans

Organism

Organism	UniProt	Comment	Textmining
Halalkalibacterium halodurans	-	and synthetic constructs	-
Halalkalibacterium halodurans C-125	-	and synthetic constructs	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	RNase (H)binding induces conformational changes in RNA-DNA hybrid. RNase H distorts the DNA strand of the hybrid by rotating the phosphodiester backbone around nucleotide dA6 about 5 A x02into the nucleotide binding pocket. The superimposition of the 2 RNA-DNA hybrids shows the RNA strand has a relatively similar conformation, but the DNA strand has been distorted to significantly widen the major groove of the helix. This flexibility of the DNA strand likely plays a role in substrate recognition and discrimination	Halalkalibacterium halodurans	?	-	?
additional information	RNase (H)binding induces conformational changes in RNA-DNA hybrid. RNase H distorts the DNA strand of the hybrid by rotating the phosphodiester backbone around nucleotide dA6 about 5 A x02into the nucleotide binding pocket. The superimposition of the 2 RNA-DNA hybrids shows the RNA strand has a relatively similar conformation, but the DNA strand has been distorted to significantly widen the major groove of the helix. This flexibility of the DNA strand likely plays a role in substrate recognition and discrimination	Halalkalibacterium halodurans C-125	?	-	?

Synonyms

Synonyms	Comment	Organism
RNase H	-	Halalkalibacterium halodurans

General Information

General Information	Comment	Organism
additional information	conformational changes induced by RNase H binding of RNA:DNA heteroduplexes, crystal structure analysis of a dodecameric nonpolypurine/polypyrimidine tract RNA-DNA duplex and of the same sequence bound to RNase H, overview. The structural changes to the duplex include widening of the major groove to 12.5 A from 4.2 A and decrease in the degree of bending along the axis which may play a crucial role in the ribonucleotide recognition and cleavage mechanism within RNase H	Halalkalibacterium halodurans
physiological function	ribonucleotides within RNA-DNA hybrids are recognized and hydrolyzed by the RNase H enzymes	Halalkalibacterium halodurans