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Literature summary for 3.1.26.4 extracted from
- An allosteric switch-based hairpin for label-free chemiluminescence detection of ribonuclease H activity and inhibitors (2019), Analyst, 144, 1420-1425 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ellipticine | - |
Homo sapiens |  |
| additional information | for screening of inhibitors, a label-free chemiluminescent (CL) aptasensor for the sensitive detection of RNase H activity based on hairpin technology is used, overview | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for catalysis | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O60930 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| A-549 cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | a label-free chemiluminescent (CL) aptasensor is used for the sensitive detection of RNase H activity based on hairpin technology. The specific hairpin structure is a DNA-RNA chimeric strand, which contains a streptavidin aptamer sequence and a blocked RNA sequence. RNase H can specifically recognize and cleave the RNA sequence of the DNA-RNA hybrid stem, liberating the streptavidin aptamer (SA, 5'-ATT GAC CGC TGT GTG ACG CAA CAC TCA AT-3') which can be accumulated by streptavidin-coated magnetic microspheres (SA-MP). Then the CL signal is generated due to an instantaneous derivatization reaction between the specific CL reagent 3,4,5-trimethoxyphenyl-glyoxal (TMPG) and the guanine (G) nucleotides in the SA aptamer, method optimization, overview | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase H1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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