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Literature summary for 3.1.26.4 extracted from

  • Permanasari, E.D.; Angkawidjaja, C.; Koga, Y.; Kanaya, S.
    Role of N-terminal extension of Bacillus stearothermophilus RNase H2 and C-terminal extension of Thermotoga maritima RNase H2 (2013), FEBS J., 280, 5065-5079.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli MIC2067(DE3) cells Geobacillus stearothermophilus
expressed in Escherichia coli MIC2067(DE3) cells Thermotoga maritima

General Stability

General Stability Organism
the C-terminal extension of the enzyme functions as a substrate-binding domain and stabilizes the RNase H domain Thermotoga maritima
the N-terminal extension of the enzyme functions as a substrate-binding domain and stabilizes the RNase H domain Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the optimum concentration is 1 mM Thermotoga maritima
Mg2+ the optimum concentration is 10 mM Geobacillus stearothermophilus
Mn2+ the optimum concentration is 1 mM Thermotoga maritima
Mn2+ the optimum concentration is 10 mM Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26630
-
1 * 26630, SDS-PAGE Thermotoga maritima
27000
-
gel filtration Thermotoga maritima
28892
-
1 * 28892, SDS-PAGE Geobacillus stearothermophilus
32000
-
gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
12 base pair RNA-DNA hybrid + H2O Geobacillus stearothermophilus
-
ribonucleotide 5'-phosphomonoester + ?
-
?
12 base pair RNA-DNA hybrid + H2O Thermotoga maritima
-
ribonucleotide 5'-phosphomonoester + ?
-
?
12 base pair RNA-DNA hybrid + H2O Thermotoga maritima DSM 3109
-
ribonucleotide 5'-phosphomonoester + ?
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Thermotoga maritima Q9X017
-
-
Thermotoga maritima DSM 3109 Q9X017
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration Geobacillus stearothermophilus
HiTrap Q column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12 base pair RNA-DNA hybrid + H2O
-
Geobacillus stearothermophilus ribonucleotide 5'-phosphomonoester + ?
-
?
12 base pair RNA-DNA hybrid + H2O
-
Thermotoga maritima ribonucleotide 5'-phosphomonoester + ?
-
?
12 base pair RNA-DNA hybrid + H2O
-
Thermotoga maritima DSM 3109 ribonucleotide 5'-phosphomonoester + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 26630, SDS-PAGE Thermotoga maritima
monomer 1 * 28892, SDS-PAGE Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
RNase H2
-
Geobacillus stearothermophilus
RNase H2
-
Thermotoga maritima
RNH2
-
Geobacillus stearothermophilus
RNH2
-
Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53.1
-
melting temperature Geobacillus stearothermophilus
66.1
-
melting temperature Thermotoga maritima