Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli MIC2067(DE3) cells | Geobacillus stearothermophilus |
expressed in Escherichia coli MIC2067(DE3) cells | Thermotoga maritima |
General Stability | Organism |
---|---|
the C-terminal extension of the enzyme functions as a substrate-binding domain and stabilizes the RNase H domain | Thermotoga maritima |
the N-terminal extension of the enzyme functions as a substrate-binding domain and stabilizes the RNase H domain | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the optimum concentration is 1 mM | Thermotoga maritima | |
Mg2+ | the optimum concentration is 10 mM | Geobacillus stearothermophilus | |
Mn2+ | the optimum concentration is 1 mM | Thermotoga maritima | |
Mn2+ | the optimum concentration is 10 mM | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26630 | - |
1 * 26630, SDS-PAGE | Thermotoga maritima |
27000 | - |
gel filtration | Thermotoga maritima |
28892 | - |
1 * 28892, SDS-PAGE | Geobacillus stearothermophilus |
32000 | - |
gel filtration | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
12 base pair RNA-DNA hybrid + H2O | Geobacillus stearothermophilus | - |
ribonucleotide 5'-phosphomonoester + ? | - |
? | |
12 base pair RNA-DNA hybrid + H2O | Thermotoga maritima | - |
ribonucleotide 5'-phosphomonoester + ? | - |
? | |
12 base pair RNA-DNA hybrid + H2O | Thermotoga maritima DSM 3109 | - |
ribonucleotide 5'-phosphomonoester + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Thermotoga maritima | Q9X017 | - |
- |
Thermotoga maritima DSM 3109 | Q9X017 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Q column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration | Geobacillus stearothermophilus |
HiTrap Q column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
12 base pair RNA-DNA hybrid + H2O | - |
Geobacillus stearothermophilus | ribonucleotide 5'-phosphomonoester + ? | - |
? | |
12 base pair RNA-DNA hybrid + H2O | - |
Thermotoga maritima | ribonucleotide 5'-phosphomonoester + ? | - |
? | |
12 base pair RNA-DNA hybrid + H2O | - |
Thermotoga maritima DSM 3109 | ribonucleotide 5'-phosphomonoester + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 26630, SDS-PAGE | Thermotoga maritima |
monomer | 1 * 28892, SDS-PAGE | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
RNase H2 | - |
Geobacillus stearothermophilus |
RNase H2 | - |
Thermotoga maritima |
RNH2 | - |
Geobacillus stearothermophilus |
RNH2 | - |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
53.1 | - |
melting temperature | Geobacillus stearothermophilus |
66.1 | - |
melting temperature | Thermotoga maritima |