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Literature summary for 3.1.26.4 extracted from

  • Alla, N.R.; Nicholson, A.W.
    Evidence for a dual functional role of a conserved histidine in RNA-DNA heteroduplex cleavage by human RNase H1 (2012), FEBS J., 279, 4492-4500.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)[pLysS] cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H264A the mutation causes an about 100fold decrease in kcat under multiple-turnover conditions, but does not alter the Km value. The H264A mutant is not rescued by increasing the Mg2+ concentration to 80 mM Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ Mg2+ is inhibitory at concentrations above 10 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000097
-
 RNA-DNA heteroduplex wild type enzyme, at pH 7.5 and 30°C Homo sapiens
0.000106
-
 RNA-DNA heteroduplex mutant enzyme H264A, at pH 7.5 and 30°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-
nucleus
-
 Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 10 mM Co2+ supports activity, with only minor inhibition observed at higher concentrations Homo sapiens
K+ optimum KCl concentration of 100-150 mM Homo sapiens
Mg2+ Mg2+ best supports the enzyme, with an optimal concentration of 10 mM Homo sapiens
Mn2+ 5 mM Mn2+ supports activity, with only minor inhibition observed at higher concentrations Homo sapiens
additional information not stimulated by Ca2+ Homo sapiens
Ni2+ exhibits only modest activity as cofactor Homo sapiens
Zn2+ exhibits only modest activity as cofactor Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RNA-DNA heteroduplex + H2O Homo sapiens
-
 ribonucleotide 5'-phosphomonoester + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap Ni-NTA column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA-DNA heteroduplex + H2O
-
 Homo sapiens ribonucleotide 5'-phosphomonoester + ?
-
 ?

Synonyms

Synonyms Comment Organism
RNase H1
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.025
-
 RNA-DNA heteroduplex mutant enzyme H264A, at pH 7.5 and 30°C Homo sapiens
3.5
-
 RNA-DNA heteroduplex wild type enzyme, at pH 7.5 and 30°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 in Hepes buffer Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.5 11 more than 60% activity at pH 6.5, about 70% activity at pH 7.0, maximum activity at pH 7.5, more than 80% activity between pH 8.0 and 11.0 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
240
-
 RNA-DNA heteroduplex mutant enzyme H264A, at pH 7.5 and 30°C Homo sapiens
36000
-
 RNA-DNA heteroduplex wild type enzyme, at pH 7.5 and 30°C Homo sapiens