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Literature summary for 3.1.26.4 extracted from

  • Kirby, K.A.; Marchand, B.; Ong, Y.T.; Ndongwe, T.P.; Hachiya, A.; Michailidis, E.; Leslie, M.D.; Sietsema, D.V.; Fetterly, T.L.; Dorst, C.A.; Singh, K.; Wang, Z.; Parniak, M.A.; Sarafianos, S.G.
    Structural and inhibition studies of the RNase H function of xenotropic murine leukemia virus-related virus reverse transcriptase (2012), Antimicrob. Agents Chemother., 56, 2048-2061.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL-21cells Xenotropic MuLV-related virus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 27% (w/v) polyethylene glycol 1500 and 0.1 M sodium citrate, pH 4.7 Xenotropic MuLV-related virus

Inhibitors

Inhibitors Comment Organism Structure
4-[(4'-aminomethyl-1,1'-biphenyl)methyl]-1-hydroxy-1,8-naphthyridin-2-one
-
 Xenotropic MuLV-related virus
7-(furan-2-yl)-2-hydroxy-isoquinoline-1,3(2H,4H)-dione YLC2-155 Xenotropic MuLV-related virus
trihydroxy benzoyl biphenyl carboxylate hydrazone BHMP07 Xenotropic MuLV-related virus
trihydroxybenzoyl naphthyl hydrazone
-
 Xenotropic MuLV-related virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ optimal RNase H activity in the presence of Mn2+ and not Mg2+ Xenotropic MuLV-related virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RNA-DNA hybrid + H2O Xenotropic MuLV-related virus
-
 ribonucleotide 5'-phosphomonoester + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Xenotropic MuLV-related virus A1Z651 clone VP62
-

Purification (Commentary)

Purification (Comment) Organism
GST column chromatography Xenotropic MuLV-related virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA-DNA hybrid + H2O
-
 Xenotropic MuLV-related virus ribonucleotide 5'-phosphomonoester + ?
-
 ?
RNA-DNA hybrid HTS-1 + H2O 5'-GAUCUGAGCCUGGGAGCU-fluorescein-3' annealed to 5'-Dabcyl-AGCTCCCAGGCTCAGATC-3' Xenotropic MuLV-related virus ?
-
 ?
RNA-DNA hybrid HTS-2 + H2O 5'-CUGGUUAGACCAGAUCUGAGCCUGGGAGCU-fluorescein-3' annealed to 5'-Dabcyl-AGCTCCCAGGCTCAGATC-3' Xenotropic MuLV-related virus ?
-
 ?

Synonyms

Synonyms Comment Organism
reverse transcriptase possesses also RNase H activity Xenotropic MuLV-related virus
RNase H
-
 Xenotropic MuLV-related virus
RNH
-
 Xenotropic MuLV-related virus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000357
-
 with RNA-DNA hybrid HTS-1 as substrate, at pH 7.8 and 37°C Xenotropic MuLV-related virus 4-[(4'-aminomethyl-1,1'-biphenyl)methyl]-1-hydroxy-1,8-naphthyridin-2-one
0.000402
-
 with RNA-DNA hybrid HTS-2 as substrate, at pH 7.8 and 37°C Xenotropic MuLV-related virus 4-[(4'-aminomethyl-1,1'-biphenyl)methyl]-1-hydroxy-1,8-naphthyridin-2-one
0.01
-
 IC50 above 0.01 mM, with RNA-DNA hybrid HTS-1 as substrate, at pH 7.8 and 37°C Xenotropic MuLV-related virus 7-(furan-2-yl)-2-hydroxy-isoquinoline-1,3(2H,4H)-dione
0.01
-
 IC50 above 0.01 mM, with RNA-DNA hybrid HTS-1 as substrate, at pH 7.8 and 37°C Xenotropic MuLV-related virus trihydroxy benzoyl biphenyl carboxylate hydrazone
0.01
-
 IC50 above 0.01 mM, with RNA-DNA hybrid HTS-1 as substrate, at pH 7.8 and 37°C Xenotropic MuLV-related virus trihydroxybenzoyl naphthyl hydrazone
0.01
-
 IC50 above 0.01 mM, with RNA-DNA hybrid HTS-2 as substrate, at pH 7.8 and 37°C Xenotropic MuLV-related virus 7-(furan-2-yl)-2-hydroxy-isoquinoline-1,3(2H,4H)-dione
0.01
-
 IC50 above 0.01 mM, with RNA-DNA hybrid HTS-2 as substrate, at pH 7.8 and 37°C Xenotropic MuLV-related virus trihydroxy benzoyl biphenyl carboxylate hydrazone
0.01
-
 IC50 above 0.01 mM, with RNA-DNA hybrid HTS-2 as substrate, at pH 7.8 and 37°C Xenotropic MuLV-related virus trihydroxybenzoyl naphthyl hydrazone