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Literature summary for 3.1.26.4 extracted from

  • Rohman, M.S.; Tadokoro, T.; Angkawidjaja, C.; Abe, Y.; Matsumura, H.; Koga, Y.; Takano, K.; Kanaya, S.
    Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses (2009), FEBS J., 276, 603-613.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
N29K/D39G/M76V/K90N/R97G/D136H mutant, to 2.5 A resolution. The main chain fold and interactions of the side-chains of the RNase HI protein are basically identical to those of the wild-type protein, except for the mutation sites. The mutations independently affect the protein structure, and the thermostabilizing effects of the mutations are roughly additive Shewanella oneidensis MR-1

Protein Variants

Protein Variants Comment Organism
D136H stabilization in melting temperature by 9.7 degrees and 6.1 kJ per mol. 65% odf wild-type activity Shewanella oneidensis MR-1
N29K/D39G/M76V/K90N stabilization in melting temperature by 18.7 degrees. 70% odf wild-type activity Shewanella oneidensis MR-1
N29K/D39G/M76V/K90N/R97G stabilization in melting temperature by 22.1 degrees. 65% of wild-type activity Shewanella oneidensis MR-1
N29K/D39G/M76V/K90N/R97G/D136H stabilization in melting temperature by 28.8 degrees. 43% of wild-type activity Shewanella oneidensis MR-1
R97G stabilization in melting temperature by 5.4 degrees and 3.5 kJ per mol. 99% of wild-type activity Shewanella oneidensis MR-1

Organism

Organism UniProt Comment Textmining
Shewanella oneidensis MR-1 Q8EE30
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4
-
mutant N29K/D39G/M76V/K90N/R97G/D136H, 30°C Shewanella oneidensis MR-1
5.1
-
mutant D136H, 30°C Shewanella oneidensis MR-1
5.1
-
mutant N29K/D39G/M76V/K90N/R97G, 30°C Shewanella oneidensis MR-1
5.5
-
mutant N29K/D39G/M76V/K90N, 30°C Shewanella oneidensis MR-1
7.7
-
mutant R97G, 30°C Shewanella oneidensis MR-1
7.8
-
wild-type, 30°C Shewanella oneidensis MR-1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA-DNA duplex + H2O
-
Shewanella oneidensis MR-1 ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30.4
-
Tm of wild-type Shewanella oneidensis MR-1
35.8
-
Tm of mutant R97G Shewanella oneidensis MR-1
40.1
-
Tm of mutant D136H Shewanella oneidensis MR-1
49.1
-
Tm of mutant N29K/D39G/M76V/K90N Shewanella oneidensis MR-1
52.5
-
Tm of mutant N29K/D39G/M76V/K90N/R97G Shewanella oneidensis MR-1
59.2
-
Tm of mutant N29K/D39G/M76V/K90N/R97G/D136H Shewanella oneidensis MR-1