Crystallization (Comment) | Organism |
---|---|
N29K/D39G/M76V/K90N/R97G/D136H mutant, to 2.5 A resolution. The main chain fold and interactions of the side-chains of the RNase HI protein are basically identical to those of the wild-type protein, except for the mutation sites. The mutations independently affect the protein structure, and the thermostabilizing effects of the mutations are roughly additive | Shewanella oneidensis MR-1 |
Protein Variants | Comment | Organism |
---|---|---|
D136H | stabilization in melting temperature by 9.7 degrees and 6.1 kJ per mol. 65% odf wild-type activity | Shewanella oneidensis MR-1 |
N29K/D39G/M76V/K90N | stabilization in melting temperature by 18.7 degrees. 70% odf wild-type activity | Shewanella oneidensis MR-1 |
N29K/D39G/M76V/K90N/R97G | stabilization in melting temperature by 22.1 degrees. 65% of wild-type activity | Shewanella oneidensis MR-1 |
N29K/D39G/M76V/K90N/R97G/D136H | stabilization in melting temperature by 28.8 degrees. 43% of wild-type activity | Shewanella oneidensis MR-1 |
R97G | stabilization in melting temperature by 5.4 degrees and 3.5 kJ per mol. 99% of wild-type activity | Shewanella oneidensis MR-1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella oneidensis MR-1 | Q8EE30 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.4 | - |
mutant N29K/D39G/M76V/K90N/R97G/D136H, 30°C | Shewanella oneidensis MR-1 |
5.1 | - |
mutant D136H, 30°C | Shewanella oneidensis MR-1 |
5.1 | - |
mutant N29K/D39G/M76V/K90N/R97G, 30°C | Shewanella oneidensis MR-1 |
5.5 | - |
mutant N29K/D39G/M76V/K90N, 30°C | Shewanella oneidensis MR-1 |
7.7 | - |
mutant R97G, 30°C | Shewanella oneidensis MR-1 |
7.8 | - |
wild-type, 30°C | Shewanella oneidensis MR-1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA-DNA duplex + H2O | - |
Shewanella oneidensis MR-1 | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30.4 | - |
Tm of wild-type | Shewanella oneidensis MR-1 |
35.8 | - |
Tm of mutant R97G | Shewanella oneidensis MR-1 |
40.1 | - |
Tm of mutant D136H | Shewanella oneidensis MR-1 |
49.1 | - |
Tm of mutant N29K/D39G/M76V/K90N | Shewanella oneidensis MR-1 |
52.5 | - |
Tm of mutant N29K/D39G/M76V/K90N/R97G | Shewanella oneidensis MR-1 |
59.2 | - |
Tm of mutant N29K/D39G/M76V/K90N/R97G/D136H | Shewanella oneidensis MR-1 |