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Literature summary for 3.1.26.4 extracted from

  • Honcharenko, D.; Barman, J.; Varghese, O.P.; Chattopadhyaya, J.
    Comparison of the RNase H cleavage kinetics and blood serum stability of the north-conformationally constrained and 2-alkoxy modified oligonucleotides (2007), Biochemistry, 46, 5635-5646.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information binding affinity of the chimeric antisense oligonucleotides to the target RNA and DNA, Michaelis-Menten kinetics, overview, dependence of the initial velocity on the substrate concentration, overview Escherichia coli
0.000064
-
DNA-RNA hybrid pH 7.5, 21°C Escherichia coli
0.000083
-
DNA-(oxetane-T)-antisense-RNA hybrid pH 7.5, 21°C Escherichia coli
0.000122
-
DNA-(1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid pH 7.5, 21°C Escherichia coli
0.000236
-
DNA-(azetidine-T)-antisense-RNA hybrid pH 7.5, 21°C Escherichia coli
0.000242
-
DNA-(2'-alkoxy-1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid pH 7.5, 21°C Escherichia coli
0.000532
-
DNA-(aza-ENA-T)-antisense-RNA hybrid pH 7.5, 21°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-(1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid + H2O
-
Escherichia coli ?
-
?
DNA-(2'-alkoxy-1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid + H2O
-
Escherichia coli ?
-
?
DNA-(aza-ENA-T)-antisense-RNA hybrid + H2O
-
Escherichia coli ?
-
?
DNA-(azetidine-T)-antisense-RNA hybrid + H2O
-
Escherichia coli ?
-
?
DNA-(oxetane-T)-antisense-RNA hybrid + H2O
-
Escherichia coli ?
-
?
DNA-RNA hybrid + H2O
-
Escherichia coli DNA + 5'-phosphonucleotides
-
?
additional information determination of RNase H cleavage potential of the RNA strand basepaired with the complementary antisense oligonucleotides containing North-East conformationally constrained 1',2'-methylene-bridged azetidine-T and oxetane-T nucleosides, North-constrained 2',4'-ethylene-bridged aza-ENA-T nucleoside, and 2'-alkoxy modified nucleosides, i.e. 2'-O-Me-T and 2'-O-MOE-T modifications, molecular dynamics, overview Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
RNase H
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
21
-
assay at Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.47
-
DNA-(oxetane-T)-antisense-RNA hybrid pH 7.5, 21°C Escherichia coli
1.54
-
DNA-RNA hybrid pH 7.5, 21°C Escherichia coli
2.13
-
DNA-(azetidine-T)-antisense-RNA hybrid pH 7.5, 21°C Escherichia coli
2.43
-
DNA-(1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid pH 7.5, 21°C Escherichia coli
2.5
-
DNA-(2'-alkoxy-1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid pH 7.5, 21°C Escherichia coli
3.97
-
DNA-(aza-ENA-T)-antisense-RNA hybrid pH 7.5, 21°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli