KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | binding affinity of the chimeric antisense oligonucleotides to the target RNA and DNA, Michaelis-Menten kinetics, overview, dependence of the initial velocity on the substrate concentration, overview | Escherichia coli | |
0.000064 | - |
DNA-RNA hybrid | pH 7.5, 21°C | Escherichia coli | |
0.000083 | - |
DNA-(oxetane-T)-antisense-RNA hybrid | pH 7.5, 21°C | Escherichia coli | |
0.000122 | - |
DNA-(1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid | pH 7.5, 21°C | Escherichia coli | |
0.000236 | - |
DNA-(azetidine-T)-antisense-RNA hybrid | pH 7.5, 21°C | Escherichia coli | |
0.000242 | - |
DNA-(2'-alkoxy-1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid | pH 7.5, 21°C | Escherichia coli | |
0.000532 | - |
DNA-(aza-ENA-T)-antisense-RNA hybrid | pH 7.5, 21°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-(1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid + H2O | - |
Escherichia coli | ? | - |
? | |
DNA-(2'-alkoxy-1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid + H2O | - |
Escherichia coli | ? | - |
? | |
DNA-(aza-ENA-T)-antisense-RNA hybrid + H2O | - |
Escherichia coli | ? | - |
? | |
DNA-(azetidine-T)-antisense-RNA hybrid + H2O | - |
Escherichia coli | ? | - |
? | |
DNA-(oxetane-T)-antisense-RNA hybrid + H2O | - |
Escherichia coli | ? | - |
? | |
DNA-RNA hybrid + H2O | - |
Escherichia coli | DNA + 5'-phosphonucleotides | - |
? | |
additional information | determination of RNase H cleavage potential of the RNA strand basepaired with the complementary antisense oligonucleotides containing North-East conformationally constrained 1',2'-methylene-bridged azetidine-T and oxetane-T nucleosides, North-constrained 2',4'-ethylene-bridged aza-ENA-T nucleoside, and 2'-alkoxy modified nucleosides, i.e. 2'-O-Me-T and 2'-O-MOE-T modifications, molecular dynamics, overview | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase H | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
21 | - |
assay at | Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.47 | - |
DNA-(oxetane-T)-antisense-RNA hybrid | pH 7.5, 21°C | Escherichia coli | |
1.54 | - |
DNA-RNA hybrid | pH 7.5, 21°C | Escherichia coli | |
2.13 | - |
DNA-(azetidine-T)-antisense-RNA hybrid | pH 7.5, 21°C | Escherichia coli | |
2.43 | - |
DNA-(1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid | pH 7.5, 21°C | Escherichia coli | |
2.5 | - |
DNA-(2'-alkoxy-1',2'-methylene-bridged azetidine-T)-antisense-RNA hybrid | pH 7.5, 21°C | Escherichia coli | |
3.97 | - |
DNA-(aza-ENA-T)-antisense-RNA hybrid | pH 7.5, 21°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |