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Literature summary for 3.1.26.4 extracted from
- Structural Requirements at the catalytic site of the heteroduplex substrate for human RNase H1 catalysis (2004), J. Biol. Chem., 279, 36317-36326.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| Endonucleolytic cleavage to a 5'-phosphomonoester | substrate structure influence on enzyme activity, substrate binding, overview | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA-RNA duplex + H2O | the enzyme cleaves RNA exclusively in a DNA-RNA heteroduplex, cleavage pattern and site specificity dependent on the substrate structure, overview | Homo sapiens | ? | - |
? |  |
| additional information | no activity with a DNA or a RNA duplex | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase H1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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