Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of several deletion mutants | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | - |
Escherichia coli | |
KCl | - |
Homo sapiens | |
KCl | - |
Saccharomyces cerevisiae | |
KCl | - |
Pyrococcus furiosus | |
Mg2+ | - |
Escherichia coli | |
Mg2+ | - |
Homo sapiens | |
Mg2+ | - |
Saccharomyces cerevisiae | |
Mg2+ | - |
Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene rnhb, type 2 enzyme, wild-type DY330 and knock-out strain | - |
Homo sapiens | - |
RNase H1 is a type 2 enzyme | - |
Pyrococcus furiosus | - |
type 2 enzyme | - |
Saccharomyces cerevisiae | - |
type 2 enzyme | - |
Purification (Comment) | Organism |
---|---|
partially | Homo sapiens |
partially | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
JURKAT cell | - |
Homo sapiens | - |
additional information | 90% purified recombinant enzyme | Pyrococcus furiosus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dsDNA oligonucleotide with a single ribose + H2O | enzyme excises misincorporated ribonucleotides in DNA | Pyrococcus furiosus | dsDNA oligonucleotide with 1 nt gap + 5'-monophosphate ribonucleotide | - |
? | |
dsDNA oligonucleotide with a single ribose + H2O | enzyme excises misincorporated ribonucleotides in DNA, enzyme places the first 5' nick, while the second 3' cut is made by FEN-1 protein | Homo sapiens | dsDNA oligonucleotide with 1 nt gap + 5'-monophosphate ribonucleotide | - |
? | |
dsDNA oligonucleotide with a single ribose + H2O | preferred substrate, enzyme excises misincorporated ribonucleotides in DNA | Escherichia coli | dsDNA oligonucleotide with 1 nt gap + 5'-monophosphate ribonucleotide | - |
? | |
dsDNA oligonucleotide with a stretch of ribonucleotides + H2O | enzyme excises misincorporated ribonucleotides in DNA | Saccharomyces cerevisiae | dsDNA oligonucleotide with 1 nt gap + 5'-monophosphate ribonucleotide | - |
? | |
dsDNA oligonucleotides with a single ribose + H2O | preferred substrate, enzyme excises misincorporated ribonucleotides in DNA, enzyme places the first 5' nick, while the second 3' cut is made by Rad27p | Saccharomyces cerevisiae | dsDNA oligonucleotides with 1 nt gap + 5'-monophosphate ribonucleotide | - |
? | |
additional information | substrate specificity | Escherichia coli | ? | - |
? | |
additional information | substrate specificity | Homo sapiens | ? | - |
? | |
additional information | substrate specificity, enzyme plays a role in the repair of misincorporated ribonucleotides rather than or in addition to processing RNA*DNA hybrid molecules | Saccharomyces cerevisiae | ? | - |
? | |
RNA*DNA hybrid + H2O | very low activity | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase H | - |
Escherichia coli |
RNase H | - |
Homo sapiens |
RNase H | - |
Saccharomyces cerevisiae |
RNase H type 2 | - |
Saccharomyces cerevisiae |
RNase H(35) | - |
Saccharomyces cerevisiae |
RNase H1 | - |
Homo sapiens |
RNase HII | - |
Escherichia coli |
RNase HII | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Saccharomyces cerevisiae |
37 | - |
assay at | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
7.5 | - |
assay at | Homo sapiens |
7.5 | - |
assay at | Saccharomyces cerevisiae |
7.5 | - |
assay at | Pyrococcus furiosus |