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Literature summary for 3.1.26.4 extracted from

  • Rydberg, B.; Game, J.
    Excision of misincorporated ribonucleotides in DNA by RNase H (type 2) and FEN-1 in cell-free extracts (2002), Proc. Natl. Acad. Sci. USA, 99, 16654-16659.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information construction of several deletion mutants Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
KCl
-
Escherichia coli
KCl
-
Homo sapiens
KCl
-
Saccharomyces cerevisiae
KCl
-
Pyrococcus furiosus
Mg2+
-
Escherichia coli
Mg2+
-
Homo sapiens
Mg2+
-
Saccharomyces cerevisiae
Mg2+
-
Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
gene rnhb, type 2 enzyme, wild-type DY330 and knock-out strain
-
Homo sapiens
-
RNase H1 is a type 2 enzyme
-
Pyrococcus furiosus
-
type 2 enzyme
-
Saccharomyces cerevisiae
-
type 2 enzyme
-

Purification (Commentary)

Purification (Comment) Organism
partially Homo sapiens
partially Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-
JURKAT cell
-
Homo sapiens
-
additional information 90% purified recombinant enzyme Pyrococcus furiosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dsDNA oligonucleotide with a single ribose + H2O enzyme excises misincorporated ribonucleotides in DNA Pyrococcus furiosus dsDNA oligonucleotide with 1 nt gap + 5'-monophosphate ribonucleotide
-
?
dsDNA oligonucleotide with a single ribose + H2O enzyme excises misincorporated ribonucleotides in DNA, enzyme places the first 5' nick, while the second 3' cut is made by FEN-1 protein Homo sapiens dsDNA oligonucleotide with 1 nt gap + 5'-monophosphate ribonucleotide
-
?
dsDNA oligonucleotide with a single ribose + H2O preferred substrate, enzyme excises misincorporated ribonucleotides in DNA Escherichia coli dsDNA oligonucleotide with 1 nt gap + 5'-monophosphate ribonucleotide
-
?
dsDNA oligonucleotide with a stretch of ribonucleotides + H2O enzyme excises misincorporated ribonucleotides in DNA Saccharomyces cerevisiae dsDNA oligonucleotide with 1 nt gap + 5'-monophosphate ribonucleotide
-
?
dsDNA oligonucleotides with a single ribose + H2O preferred substrate, enzyme excises misincorporated ribonucleotides in DNA, enzyme places the first 5' nick, while the second 3' cut is made by Rad27p Saccharomyces cerevisiae dsDNA oligonucleotides with 1 nt gap + 5'-monophosphate ribonucleotide
-
?
additional information substrate specificity Escherichia coli ?
-
?
additional information substrate specificity Homo sapiens ?
-
?
additional information substrate specificity, enzyme plays a role in the repair of misincorporated ribonucleotides rather than or in addition to processing RNA*DNA hybrid molecules Saccharomyces cerevisiae ?
-
?
RNA*DNA hybrid + H2O very low activity Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
RNase H
-
Escherichia coli
RNase H
-
Homo sapiens
RNase H
-
Saccharomyces cerevisiae
RNase H type 2
-
Saccharomyces cerevisiae
RNase H(35)
-
Saccharomyces cerevisiae
RNase H1
-
Homo sapiens
RNase HII
-
Escherichia coli
RNase HII
-
Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli
37
-
assay at Homo sapiens
37
-
assay at Saccharomyces cerevisiae
37
-
assay at Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli
7.5
-
assay at Homo sapiens
7.5
-
assay at Saccharomyces cerevisiae
7.5
-
assay at Pyrococcus furiosus