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Literature summary for 3.1.26.4 extracted from
- Bacteriophage T4 RNase H removes both RNA primers and adjacent DNA from the 5' end of lagging strand fragments (2001), J. Biol. Chem., 276, 28516-28524.
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNA-DNA*DNA hybrid + H2O | Tequatrovirus T4 | enzyme removes RNA primers from lagging strand fragments during DNA replication, 5'-to 3'-exonuclease activity, degradation of the RNA portion of the duplex | DNA*DNA + 5'-phosphomononucleotides | - |
? |  |
| ssDNA-dsDNA + H2O | Tequatrovirus T4 | 5'-to 3'-exonuclease activity, exonuclease activity removing short oligonucleotides of 3-30 nucleotides from adjacent DNA | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tequatrovirus T4 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA*DNA + H2O | 5'-to 3'-exonuclease activity, degradation of DNA*DNA duplexes | Tequatrovirus T4 | ? | - |
? | |
| additional information | regulation | Tequatrovirus T4 | ? | - |
? | |
| RNA*DNA hybrid + H2O | 5'-to 3'-exonuclease activity, degradation of RNA*DNA duplexes | Tequatrovirus T4 | DNA + 5'-phosphonucleotides | - |
? | |
| RNA-DNA*DNA hybrid + H2O | enzyme removes RNA primers from lagging strand fragments during DNA replication, 5'-to 3'-exonuclease activity, degradation of the RNA portion of the duplex | Tequatrovirus T4 | DNA*DNA + 5'-phosphomononucleotides | - |
? | |
| RNA-DNA*DNA hybrid + H2O | specific removal of the RNA portion of the duplex | Tequatrovirus T4 | DNA*DNA + 5'-phosphomononucleotides | - |
? | |
| ssDNA-dsDNA + H2O | 5'-to 3'-exonuclease activity, exonuclease activity removing short oligonucleotides of 3-30 nucleotides from adjacent DNA | Tequatrovirus T4 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase H | - |
Tequatrovirus T4 |
| T4 RNase H | - |
Tequatrovirus T4 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Tequatrovirus T4 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Tequatrovirus T4 |
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Release 2023.1 (January 2023)
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