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Literature summary for 3.1.26.4 extracted from

  • Mangos, M.M.; Min, K.L.; Viazovkina, E.; Galarneau, A.; Elzagheid, M.I.; Parniak, M.A.; Damha, M.J.
    Efficient RNase H-directed cleavage of RNA promoted by antisense DNA or 2'F-ANA constructs containing acyclic nucleotide inserts (2003), J. Am. Chem. Soc., 125, 654-661.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
KCl activates Escherichia coli
KCl activates Homo sapiens
Mg2+ required Escherichia coli
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RNA*DNA hybrid + H2O Escherichia coli cleaves the RNA portion ?
-
?
RNA*DNA hybrid + H2O Homo sapiens cleaves the RNA portion ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
type 1 enzyme
-
Homo sapiens
-
type 2 enzyme
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate characterization and substrate specificity Homo sapiens ?
-
?
additional information substrate characterizationa and substrate specificity Escherichia coli ?
-
?
RNA*2'F-ANA-DNA hybrid + H2O cleaves the RNA portion of hybrid duplexes of butyl-modified 2'F-ANA-DNA oligonucleotides containing acyclic interresidue units with complementary RNA Escherichia coli ?
-
?
RNA*2'F-ANA-DNA hybrid + H2O cleaves the RNA portion of hybrid duplexes of butyl-modified 2'F-ANA-DNA oligonucleotides containing acyclic interresidue units with complementary RNA Homo sapiens ?
-
?
RNA*antisense-DNA hybrid + H2O cleaves the RNA portion of hybrid duplexes of modified antisense DNA oligonucleotides containing acyclic interresidue units with complementary RNA Escherichia coli ?
-
?
RNA*antisense-DNA hybrid + H2O cleaves the RNA portion of hybrid duplexes of modified antisense DNA oligonucleotides containing acyclic interresidue units with complementary RNA Homo sapiens ?
-
?
RNA*DNA hybrid + H2O cleaves the RNA portion Escherichia coli ?
-
?
RNA*DNA hybrid + H2O cleaves the RNA portion Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
RNase HI
-
Escherichia coli
RNase HII
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
about, assay at room temperature Escherichia coli
22
-
about, assay at room temperature Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Escherichia coli
additional information
-
additional information
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Escherichia coli
7.8
-
assay at Homo sapiens