Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | in presence of Mg2+, inhibition | Homo sapiens | |
Cu2+ | in presence of Mg2+, inhibition | Homo sapiens | |
Fe2+ | in presence of Mg2+, inhibition | Homo sapiens | |
Mn2+ | in presence of Mg2+, strong inhibition | Homo sapiens | |
NEM | 2 mM, 50% inhibition | Homo sapiens | |
Zn2+ | in presence of Mg2+, inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
DNA/RNA hybrid | obtained by transcription of calf thymus DNA | Homo sapiens | |
0.0016 | - |
poly(rAdT) | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens | |
Mg2+ | broad optimum around 20 mM | Homo sapiens | |
Mn2+ | slightly active with Mn2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA/RNA hybrid + H2O | - |
Homo sapiens | ? | - |
? | |
poly(rAdT) + H2O | - |
Homo sapiens | ? | - |
? | |
RNA-DNA hybrid + H2O | hybrid obtained by transcription of calf thymus DNA | Homo sapiens | ribonucleotide 5'-phosphomonoester | oligoribonucleotides with 3'-OH and 5'-phosphate ends | ? | |
RNA-DNA hybrid + H2O | poly(rAdT) | Homo sapiens | ribonucleotide 5'-phosphomonoester | oligoribonucleotides with 3'-OH and 5'-phosphate ends | ? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10.5 | pH 7.0: about 50% of maximal activity, pH 10.5: about 60% of maximal activity | Homo sapiens |