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Literature summary for 3.1.26.4 extracted from

  • Cooper, R.J.; Duff, P.M.; Craig, O.R.K.; Keir, H.M.
    Multiple ribonuclease H activities from BHK-21-C13 cells (1974), FEBS Lett., 45, 38-43.
    View publication on PubMed
Search for more literature for 3.1.26.4

Inhibitors

Inhibitors Comment Organism Structure
KCl enzyme form HA1, HA2, and HB1 Mesocricetus auratus
NEM 2 mM or above Mesocricetus auratus
S-adenosylmethionine 2 mM or above Mesocricetus auratus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl stimulates enzyme HB2 Mesocricetus auratus
Mg2+ optimal concentrations for the 4 enzyme forms at pH 7.6 and at pH 8.3 Mesocricetus auratus
Mn2+ optimal concentrations for the 4 enzyme forms at pH 7.6 and at pH 8.3 Mesocricetus auratus

Organism

Organism UniProt Comment Textmining
Mesocricetus auratus
-
 4 enzyme form: HA1, HA2, HB1 and HB2
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney BHK-21/C13 Mesocricetus auratus
-

Storage Stability

Storage Stability Organism
-70°C, complete loss of activity after a few days Mesocricetus auratus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA-DNA hybrid + H2O
-
 Mesocricetus auratus ribonucleotide 5'-phosphomonoester
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 8.8 in presence of 0.5 mM Mn2+, enzyme form HA1 Mesocricetus auratus
7.5
-
 in presence of 40 mM Mg2+, enzyme form HB2 Mesocricetus auratus
7.6
-
 in presence of 0.5 mM Mn2+, enzyme form HA2, HB1 and HB2 Mesocricetus auratus
7.7
-
 in presence of 40 mM Mg2+, enzyme form HB1 Mesocricetus auratus
8.3
-
 in presence of 5 mM Mg2+, enzyme form HA1 Mesocricetus auratus
8.6
-
 in presence of 5 mM Mg2+, enzyme form HA2 Mesocricetus auratus