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Literature summary for 3.1.26.4 extracted from

  • Sawai, Y.; Uchida, S.; Saito, J.; Sugano, N.; Tsukada, K.
    Two ribonucleases H from cultured plant cells (1979), J. Biochem., 85, 1301-1308.
    View publication on PubMed
Search for more literature for 3.1.26.4

Activating Compound

Activating Compound Comment Organism Structure
(NH4)2SO4 30% activation of Mg2+-dependent enzyme Daucus carota
sulfhydryl reagent
-
 Daucus carota

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 200 mM, 80% inhibition of Mn2+-dependent enzyme Daucus carota

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+-dependent enzyme requires 10-15 mM Mg2+ for optimal activity Daucus carota
Mn2+ 0.4 mM Mn2+ required for optimal activity, some activity with Mg2+ Daucus carota

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 Mn2+-dependent enzyme, gel filtration Daucus carota
180000
-
 Mg2+-dependent enzyme, gel filtration Daucus carota

Organism

Organism UniProt Comment Textmining
Daucus carota
-
-
-
Daucus carota
-
 one Mn2+-dependent RNase H and one Mg2+-dependent RNase H
-
Daucus carota GD-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
one Mn2+-dependent RNase H and one Mg2+-dependent RNase H Daucus carota

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Daucus carota
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA-DNA hybrid + H2O poly(rAdT) Daucus carota ribonucleotide 5'-phosphomonoester a mixture of oligoribonucleotides with 5'-phosphate and 3'-hydroxyl terminus ?
RNA-DNA hybrid + H2O poly(rAdT) Daucus carota ribonucleotide 5'-phosphomonoester oligonucleotides of various chain length, mainly 3-9 nucleotides in length ?
RNA-DNA hybrid + H2O poly(rAdT) Daucus carota GD-2 ribonucleotide 5'-phosphomonoester a mixture of oligoribonucleotides with 5'-phosphate and 3'-hydroxyl terminus ?
RNA-DNA hybrid + H2O poly(rAdT) Daucus carota GD-2 ribonucleotide 5'-phosphomonoester oligonucleotides of various chain length, mainly 3-9 nucleotides in length ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 Mn2+-dependent enzyme Daucus carota
9
-
 Mg2+-dependent enzyme Daucus carota