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Literature summary for 3.1.26.3 extracted from

  • Xu, W.; Liu, Y.; Liu, Y.; Liu, L.; Zhan, Y.; Zhuang, C.; Lin, J.; Chen, M.; Li, J.; Cai, Z.; Huang, W.; Zhang, Y.
    Artificial small RNA for sequence specific cleavage of target RNA through RNase III endonuclease Dicer (2016), Oncotarget, 7, 54549-54554 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information an artificial small RNA (asRNA), composed of a Dicer-binding RNA element and an antisense RNA, can be used to induce Dicer to process and degrade a specific RNA. Development of a method which is named DICERi for gene silencing or RNA editing. To prove the feasibility of asRNA, MALAT-1 is selected as target and Hela and MDA-MB-231 cells are used as experimental models. The results of qRT-PCR show that the introduction of asRNA decreases the relative expression level of target gene significantly. Cell proliferation and cell migration are both suppressed remarkably after asRNA is expressed in Hela and MDA-MB-231 cells. T gene silencing effects were caused by Dicer. When the cleavage role of Dicer is silenced, the relative expression level of MALAT-1 is not affected after the introduction of asRNA. The effect of asRNA is dependent on Dicer. Method evaluation, overview Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UPY3
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Dicer protein binds to target RNA through an asRNA and induces the cleavage of target RNA Homo sapiens ?
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Synonyms

Synonyms Comment Organism
Dicer
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Homo sapiens
RNase III endonuclease
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Homo sapiens