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Literature summary for 3.1.26.3 extracted from
- RNase III mediated cleavage of the coding region of mraZ mRNA is required for efficient cell division in Corynebacterium glutamicum (2016), Mol. Microbiol., 99, 1149-1166 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene rnc or cgR_1959, quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis | Corynebacterium glutamicum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of a deletion mutant of gene cgR_1959, the DELTArnc mutant. Deletion of the rnc gene encoding RNase III results in cell elongation in Corynebacterium glutamicum strain R. Microarray analysis and quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis show that the level of mraZ mRNA increases, whereas cgR_1596 and ftsEX mRNA are decreased in the DELTArnc mutant. RNase III cleaves the coding region of mraZ mRNA. Deletion of cgR_1596 caused a defect in cell separation. Phenotype, overview | Corynebacterium glutamicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| mraZ mRNA + H2O | Corynebacterium glutamicum | the degradation of mraZ mRNA is performed by RNase III and the 3'-to-5' exoribonuclease, PNPase. The cleavage site for mraZ mRNA by RNase III is in the coding region | ? | - |
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Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | A4QFE0 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| mraZ mRNA + H2O | the degradation of mraZ mRNA is performed by RNase III and the 3'-to-5' exoribonuclease, PNPase. The cleavage site for mraZ mRNA by RNase III is in the coding region | Corynebacterium glutamicum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cgR_1959 | - |
Corynebacterium glutamicum |
| RNase III | - |
Corynebacterium glutamicum |
| rnc | - |
Corynebacterium glutamicum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | deletion of cgR_1596 causes a defect in cell separation, deletion mutant of gene cgR_1959 (DELTArnc mutant) shows an elongated cell shape, and presence of several lines on the cell surface, indicating a required of RNase III for maintaining normal cell morphology in Corynebacterium glutamicum, phenotype, overview. The level of mraZ mRNA is increased, whereas cgR_1596 mRNA encoding a putative cell wall hydrolase and ftsEX mRNA is decreased in the DELTArnc mutant. The half-life of mraZ mRNA is significantly prolonged in the DELTArnc and the DELTApnp mutant strains | Corynebacterium glutamicum |
| physiological function | RNase III mediated cleavage of the coding region of mraZ mRNA is required for efficient cell division in Corynebacterium glutamicum. MraZ is a transcriptional repressor of ftsEX in Corynebacterium glutamicum. Degradation of mraZ mRNA is performed by RNase III and the 3'-to-5' exoribonuclease, PNPase. Overproduction of MraZ results in an elongated cell shape. RNase III is required for efficient expression of MraZ-dependent ftsEX and MraZ-independent cgR_1596 | Corynebacterium glutamicum |
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