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Literature summary for 3.1.26.3 extracted from
- Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules (2011), Nucleic Acids Res., 39, 5299-5311.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of GFP-tagged Rnt1p in Saccharomyces cerevisiae strain W303 from plasmid pCS321 | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | building a class of RNA sensing actuation devices based on direct integration of RNA aptamers into a region of the RNase III Rnt1p hairpin that modulates Rnt1p cleavage rates, design of an Rnt1p switch platform based on direct replacement of the CEB with an aptamer sequence. Integration of a sensor component, DELTATCT-4 aptamer, into the actuator component, R31L-3B4Inv Rnt1p hairpin. Ligand binding to the integrated aptamer domain is associated with a structural change sufficient to inhibit Rnt1p processing, overview. Three tuning strategies based on the incorporation of different functional modules into the Rnt1p switch platform optimize switch dynamics and ligand responsiveness. Application of multiple switch modules decreases theophylline responsiveness and increases fold-change. The tuning modules can be implemented combinatorially in a predictable manner to further improve the regulatory response properties of the switch. The modularity and tunability of the Rnt1p switch platform will allow for rapid optimization and tailoring of this gene control device. Method evaluation and system stabililty, overview | Saccharomyces cerevisiae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | required | Saccharomyces cerevisiae |  |
| Mg2+ | required | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase III | - |
Saccharomyces cerevisiae |
| Rnt1p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Saccharomyces cerevisiae |
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