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Literature summary for 3.1.26.3 extracted from
- Mini-III, an unusual member of the RNase III family of enzymes, catalyses 23S ribosomal RNA maturation in B. subtilis (2008), Mol. Microbiol., 68, 1096-1106.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | DELTAmrnC strain has no major difference in growth rate compared with wild-type, but reaches a slightly lower saturation density | Bacillus subtilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17100 | - |
2 * 17100, His-tagged monomer, calculated | Bacillus subtilis |
| 38100 | - |
gel filtration | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bacillus subtilis | mini-III contains an RNase III-like catalytic domain, but curiously lacks the double-stranded RNA binding domain typical of RNase III itself, Dicer, Drosha and other well-known members of this family of enzymes | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | O31418 | strain W168 and strain yazCdd (SSB1044) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration analysis on a Superdex 75 10/30 column | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dsRNA + H2O | - |
Bacillus subtilis | mature RNA | - |
? | |
| additional information | mini-III contains an RNase III-like catalytic domain, but curiously lacks the double-stranded RNA binding domain typical of RNase III itself, Dicer, Drosha and other well-known members of this family of enzymes | Bacillus subtilis | ? | - |
? | |
| pre-23S rRNA + H2O | - |
Bacillus subtilis | mature 23S rRNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 17100, His-tagged monomer, calculated | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mini-III | unusual member of the RNase III family of enzymes, catalyses 23S ribosomal RNA maturation in Bacillus subtilis. Defines a fourth class of naturally occurring functional RNase III enzymes, which consists of just the catalytic domain | Bacillus subtilis |
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