Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BLR(DE3) cells | Escherichia coli |
expression in Escherichia coli BL21(DE3)rnc105,recA | Aquifex aeolicus |
expression in Escherichia coli BL21(DE3)rnc105,recA | Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
Aquifex aeolicus RNase III also has been cocrystallized with dsRNA or specific hairpin substrates | Aquifex aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O67082 | - |
- |
Escherichia coli | - |
- |
- |
Thermotoga maritima | Q9X0I6 | - |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography. RNase III heterodimers also can be purified from the inclusion body | Escherichia coli |
Ni2+ affinity column | Aquifex aeolicus |
Ni2+ affinity column | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded RNA + H2O | - |
Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
double-stranded RNA + H2O | - |
Aquifex aeolicus | 5'-phosphooligonucleotides | - |
? | |
double-stranded RNA + H2O | - |
Thermotoga maritima | 5'-phosphooligonucleotides | - |
? | |
additional information | cleave internally 32P-labeled R1.1(WC) RNA | Aquifex aeolicus | ? | - |
? | |
additional information | cleaves internally 32P-labeled R1.1(WC) RNA | Escherichia coli | ? | - |
? | |
additional information | cleaves internally 32P-labeled R1.1(WC) RNA | Thermotoga maritima | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | - |
Aquifex aeolicus |
heterodimer | preparation of artificial heterodimers of RNase III, which are providing new insight on the subunit and domain interactions involved in dsRNA recognition and cleavage | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
Aa-RNase III | - |
Aquifex aeolicus |
ribonuclease III | - |
Escherichia coli |
ribonuclease III | - |
Aquifex aeolicus |
ribonuclease III | - |
Thermotoga maritima |
RNase III | - |
Escherichia coli |
RNase III | - |
Aquifex aeolicus |
RNase III | - |
Thermotoga maritima |
Tm-RNase III | - |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
hyperthermophilic bacterium | Aquifex aeolicus |
additional information | - |
hyperthermophilic bacterium | Thermotoga maritima |