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Literature summary for 3.1.26.3 extracted from
- A ribonuclease III domain protein functions in group II intron splicing in maize chloroplasts (2007), Plant Cell, 19, 2606-2623.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pMAL-c2X vector, containing a TEV protease cleavage site at the EcoRI site, for expression in Escherichia coli Rosetta 2 cells | Zea mays |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Zea mays | 9507 | - |
| chloroplast stroma | - |
Zea mays | 9570 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | A6YSL1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified by affinity chromatography on amylose-coupled agarose resin, recombinant RNC1 binds RNA but lacks endonuclease activity | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Zea mays | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 40 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM EDTA, 5 mM 2-mercaptoethanol, 50% glycerol | Zea mays |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribonuclease III | - |
Zea mays |
| RNase III | - |
Zea mays |
| RNC1 | plant specific protein that contains two RNase III domains | Zea mays |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
endonuclease assay | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
endonuclease assay | Zea mays |
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Release 2023.1 (January 2023)
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