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Literature summary for 3.1.26.3 extracted from

  • Du, Z.; Lee, J.K.; Tjhen, R.; Stroud, R.M.; James, T.L.
    Structural and biochemical insights into the dicing mechanism of mouse Dicer: a conserved lysine is critical for dsRNA cleavage (2008), Proc. Natl. Acad. Sci. USA, 105, 2391-2396.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.3

Cloned(Commentary)

Cloned (Comment) Organism
Dicer fragment RNase IIIb + dsRBD expressed as His-tag fusion protein in Escherichia coli BL21(DE3) Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
handing drop vapor diffusion method, crystallization of a catalytically active fragment of mouse Dicer, containing the RNase IIIb and dsRNA binding domains, in its apo and Cd2+-bound forms Mus musculus

Protein Variants

Protein Variants Comment Organism
K1790A significantly reduced activity Mus musculus
K1790R significantly reduced activity Mus musculus
K1790S significantly reduced activity Mus musculus
K1790T significantly reduced activity Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded RNA + H2O Mus musculus processing of precursor dsRNAs into mature microRNAs and small-interfering RNAs 5'-phosphooligonucleotides
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 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q8R418
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-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme using His-tag Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded RNA + H2O processing of precursor dsRNAs into mature microRNAs and small-interfering RNAs Mus musculus 5'-phosphooligonucleotides
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 ?
double-stranded RNA + H2O in vitro dsRNA cleavage assay with a designed 52-nt stem-loop RNA containing a 24-bp stem capped by a GCAA tetraloop as the substrate Mus musculus 5'-phosphooligonucleotides
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 ?

Subunits

Subunits Comment Organism
dimer the RNase IIIb + dsRBD construct exists as a symmetric homodimer, crystal structure analysis and gel filtration, dimer is catalytically active Mus musculus

Synonyms

Synonyms Comment Organism
Dicer enzyme complex containing RNAse III enzyme Mus musculus