Cloned (Comment) | Organism |
---|---|
cDNA fragment encoding amino acids 1-391, expression as His-tag fusion protein | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Arabidopsis thaliana | 5737 | - |
nucleus | - |
Arabidopsis thaliana | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, calculated from the deduced amino acid sequence for AtRTL1 | Arabidopsis thaliana |
45000 | - |
2 * 45000, subunit mass calculated from the deduced amino acid sequence for AtRTL2, native mass by gel filtration, forms highly salt resistant dimers, dimer formation through disulfide bonds, dimers can be disrupted upon DTT treatment | Arabidopsis thaliana |
108000 | - |
x * 108000, calculated from the deduced amino acid sequence for AtRTL3 | Arabidopsis thaliana |
110000 | - |
gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded RNA + H2O | Arabidopsis thaliana | responsible for processing and maturation of RNA precursors into functional rRNA, mRNA and other small RNA | 5'-phosphooligonucleotides | - |
? | |
additional information | Arabidopsis thaliana | required for 3external transcribed spacer (ETS) cleavage of the pre-rRNA in vivo | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
Columbia Col 0 ecotype, three different proteins named AtRTL1-AtRTL3 | - |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Arabidopsis thaliana |
Renatured (Comment) | Organism |
---|---|
renaturation by stepwise removal of urea by dialysis | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | buds, AtRTL2 | Arabidopsis thaliana | - |
leaf | AtRTL2 | Arabidopsis thaliana | - |
root | AtRTL1 and AtRTL2 | Arabidopsis thaliana | - |
seed | dry and germinating, AtRTL2 | Arabidopsis thaliana | - |
seedling | AtRTL2 | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded RNA + H2O | responsible for processing and maturation of RNA precursors into functional rRNA, mRNA and other small RNA | Arabidopsis thaliana | 5'-phosphooligonucleotides | - |
? | |
additional information | required for 3external transcribed spacer (ETS) cleavage of the pre-rRNA in vivo | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 108000, calculated from the deduced amino acid sequence for AtRTL3 | Arabidopsis thaliana |
? | x * 33000, calculated from the deduced amino acid sequence for AtRTL1 | Arabidopsis thaliana |
dimer | 2 * 45000, subunit mass calculated from the deduced amino acid sequence for AtRTL2, native mass by gel filtration, forms highly salt resistant dimers, dimer formation through disulfide bonds, dimers can be disrupted upon DTT treatment | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtRTL1 | - |
Arabidopsis thaliana |
AtRTL2 | - |
Arabidopsis thaliana |
AtRTL3 | - |
Arabidopsis thaliana |
AtRTL3 | protein not detected in any of the tissues studied, possibly non-functional gene sequence | Arabidopsis thaliana |