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Literature summary for 3.1.26.3 extracted from

  • Comella, P.; Pontvianne, F.; Lahmy, S.; Vignols, F.; Barbezier, N.; Debures, A.; Jobet, E.; Brugidou, E.; Echeverria, M.; Saez-Vasquez, J.
    Characterization of a ribonuclease III-like protein required for cleavage of the pre-rRNA in the 3ETS in Arabidopsis (2008), Nucleic Acids Res., 36, 1163-1175.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cDNA fragment encoding amino acids 1-391, expression as His-tag fusion protein Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Arabidopsis thaliana 5737
-
nucleus
-
Arabidopsis thaliana 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
x * 33000, calculated from the deduced amino acid sequence for AtRTL1 Arabidopsis thaliana
45000
-
2 * 45000, subunit mass calculated from the deduced amino acid sequence for AtRTL2, native mass by gel filtration, forms highly salt resistant dimers, dimer formation through disulfide bonds, dimers can be disrupted upon DTT treatment Arabidopsis thaliana
108000
-
x * 108000, calculated from the deduced amino acid sequence for AtRTL3 Arabidopsis thaliana
110000
-
gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded RNA + H2O Arabidopsis thaliana responsible for processing and maturation of RNA precursors into functional rRNA, mRNA and other small RNA 5'-phosphooligonucleotides
-
?
additional information Arabidopsis thaliana required for 3’external transcribed spacer (ETS) cleavage of the pre-rRNA in vivo ?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
Columbia Col 0 ecotype, three different proteins named AtRTL1-AtRTL3
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Arabidopsis thaliana

Renatured (Commentary)

Renatured (Comment) Organism
renaturation by stepwise removal of urea by dialysis Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower buds, AtRTL2 Arabidopsis thaliana
-
leaf AtRTL2 Arabidopsis thaliana
-
root AtRTL1 and AtRTL2 Arabidopsis thaliana
-
seed dry and germinating, AtRTL2 Arabidopsis thaliana
-
seedling AtRTL2 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded RNA + H2O responsible for processing and maturation of RNA precursors into functional rRNA, mRNA and other small RNA Arabidopsis thaliana 5'-phosphooligonucleotides
-
?
additional information required for 3’external transcribed spacer (ETS) cleavage of the pre-rRNA in vivo Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
? x * 108000, calculated from the deduced amino acid sequence for AtRTL3 Arabidopsis thaliana
? x * 33000, calculated from the deduced amino acid sequence for AtRTL1 Arabidopsis thaliana
dimer 2 * 45000, subunit mass calculated from the deduced amino acid sequence for AtRTL2, native mass by gel filtration, forms highly salt resistant dimers, dimer formation through disulfide bonds, dimers can be disrupted upon DTT treatment Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AtRTL1
-
Arabidopsis thaliana
AtRTL2
-
Arabidopsis thaliana
AtRTL3
-
Arabidopsis thaliana
AtRTL3 protein not detected in any of the tissues studied, possibly non-functional gene sequence Arabidopsis thaliana