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Literature summary for 3.1.26.3 extracted from

  • Calin-Jageman, I.; Amarasinghe, A.K.; Nicholson, A.W.
    Ethidium-dependent uncoupling of substrate binding and cleavage by Escherichia coli ribonuclease III (2001), Nucleic Acids Res., 29, 1915-1925.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.3

Protein Variants

Protein Variants Comment Organism
E117Q mutant enzyme can still bind to the substrate RNA in presence of Mg2+ but cannot cleave it Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Ethidium bromide reversible, competitive inhibition in vitro, uncouples small RNA substrate binding and cleavage, intercalation inhibition mechanism, perturbation of substrate recognition by the N-terminal catalytic domain, 81% inhibition at 0.02 mM, tRNA abolishes the inhibitory effect Escherichia coli
tRNA inhibits enzyme activity and inhibition by ethidium bromide Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information substrate binding kinetics, wild-type and mutant enzyme, in presence or absence of ethidium bromide Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ds-rRNA + H2O double-strand RNA specific endonuclease Escherichia coli mature ds-rRNA
-
 ?
R1.1 RNA + H2O based on the R1.1 processing signal, which is encoded in the phage T7 genetic early region between genes 1.0 and 1.1, substrate possesses 2 ethidium bromide binding sites in the internal loop and the lower stem, respectively, both consisting of an A-A pair stacked on a CG pair, which is a motif that is a favourable environment for intercalation Escherichia coli fragments of R1.1 RNA
-
 ?

Synonyms

Synonyms Comment Organism
RNase III
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0017
-
 Ethidium bromide pH 8.0, 37°C Escherichia coli