Protein Variants | Comment | Organism |
---|---|---|
E117Q | mutant enzyme can still bind to the substrate RNA in presence of Mg2+ but cannot cleave it | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ethidium bromide | reversible, competitive inhibition in vitro, uncouples small RNA substrate binding and cleavage, intercalation inhibition mechanism, perturbation of substrate recognition by the N-terminal catalytic domain, 81% inhibition at 0.02 mM, tRNA abolishes the inhibitory effect | Escherichia coli | |
tRNA | inhibits enzyme activity and inhibition by ethidium bromide | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate binding kinetics, wild-type and mutant enzyme, in presence or absence of ethidium bromide | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ds-rRNA + H2O | double-strand RNA specific endonuclease | Escherichia coli | mature ds-rRNA | - |
? | |
R1.1 RNA + H2O | based on the R1.1 processing signal, which is encoded in the phage T7 genetic early region between genes 1.0 and 1.1, substrate possesses 2 ethidium bromide binding sites in the internal loop and the lower stem, respectively, both consisting of an A-A pair stacked on a CG pair, which is a motif that is a favourable environment for intercalation | Escherichia coli | fragments of R1.1 RNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase III | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
Ethidium bromide | pH 8.0, 37°C | Escherichia coli |