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Literature summary for 3.1.26.3 extracted from

  • Lamontagne, B.; Ghazal, G.; Lebars, I.; Yoshizawa, S.; Fourmy, D.; Abou Elela, S.
    Sequence dependence of substrate recognition and cleavage by yeast RNase III (2003), J. Mol. Biol., 327, 985-1000.
    View publication on PubMed
Search for more literature for 3.1.26.3

Inhibitors

Inhibitors Comment Organism Structure
additional information substrate binding is inhibited by a specific sequence within the dsRNA stem, overview Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, substrate binding with diverse RNA substrates Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae enzyme is involved in RNA processing and RNA interference, i.e. RNAi, regulation by a combination of primary and tertiary structural elements allowing a substrate-specific binding and cleavage efficiency ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dsRNA + H2O diverse model RNA substreates, enzyme cleaves specifically RNA stems capped with the conserved AGNN tetraloop, the dsRNA sequence adjacent to the tetraloop regulates enzyme activity by interfering with substrate binding, sequences surrounding the cleavage site directly influence the cleavage efficiency, a minimum substrate length is required Saccharomyces cerevisiae mature RNA
-
 ?
additional information enzyme is involved in RNA processing and RNA interference, i.e. RNAi, regulation by a combination of primary and tertiary structural elements allowing a substrate-specific binding and cleavage efficiency Saccharomyces cerevisiae ?
-
 ?
additional information substrate specificity, overview, RNA substrate with introduced sequences stabilizing the RNA helix enhances binding while the turnover rate is reduced, thus substrate binding becomes rate-limiting Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the double-stranded RNA specific RNase III family of endoribonucleases Saccharomyces cerevisiae
RNase III
-
 Saccharomyces cerevisiae
Rnt1p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharomyces cerevisiae