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Literature summary for 3.1.26.3 extracted from

  • Sun, W.; Jun, E.; Nicholson, A.W.
    Intrinsic double-stranded-RNA processing activity of Escherichia coli ribonuclease III lacking the dsRNA-binding domain (2001), Biochemistry, 40, 14976-14984.
    View publication on PubMed
Search for more literature for 3.1.26.3

Cloned(Commentary)

Cloned (Comment) Organism
gene rnc, expression of wild-type and mutant enzymes in strain BL21(DE3) as His-tagged enzymes Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information construction of a mutant enzyme RNase III[DELTAdsRBD] lacking the dsRNA binding domain, the mutant is still catalytically active at low salt concentrations in presence of either 25 mM Mg2+ or 5 mM Mn2+ with slightly reduced catalytic efficiency, but unaltered substrate specificity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Ethidium bromide inhibition of wild-type and deletion mutant, intercalative mode of inhibition, competitive, 50% inhibition at 0.01 mM Escherichia coli
Mn2+ inhibition of wild-type and deletion mutant at high concentration due to metal ion occupancy at an inhibitory site of the enzyme, the deletion mutant is less sensitive than the wild-type enzyme Escherichia coli
additional information enzyme is inhibited by Watson-Crick bp antideterminants, overview Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00011
-
 R1.1 RNA recombinant wild-type enzyme, 37°C, in presence of 1 mM Mn2+ Escherichia coli
0.00027
-
 R1.1 RNA recombinant enzyme mutant RNase III[DELTAdsRBD], 37°C, in presence of 5 mM Mn2+ Escherichia coli
0.00034
-
 R1.1 RNA recombinant wild-type enzyme, 37°C, in presence of 5 mM Mg2+ Escherichia coli
0.003
-
 R1.1 RNA recombinant enzyme mutant RNase III[DELTAdsRBD], 37°C, in presence of 25 mM Mg2+ Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on, optimal at 25 mM Escherichia coli
Mn2+ can substitute for Mg2+ in vitro, at 5 mM Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dsRNA + H2O Escherichia coli enzyme plays a key role in diverse maturation and degradation processes mature RNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
Endonucleolytic cleavage to a 5'-phosphomonoester Glu117 is essential for phosphodiester hydrolysis but not for substrate binding, the dsRNA-binding domain is important for substrate binding but not for catalytic activity, while the catalytic domain is important for catalytic activity but not for substrate binding Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dsRNA + H2O enzyme plays a key role in diverse maturation and degradation processes Escherichia coli mature RNA
-
 ?
dsRNA + H2O double-strand RNA-specific, the dsRNA-binding domain is important for substrate binding but not for catalytic activity, while the catalytic domain is important for catalytic activity but not for substrate binding Escherichia coli mature RNA
-
 ?
additional information no activity with (rA)25, (rU)25, (rC)25, dsDNA, ssDNA and an RNA-DNA hybrid Escherichia coli ?
-
 ?
R1.1 RNA + H2O substrate is enzymatically synthesized based on the R1.1 processing signal, which is encoded in the phage T7 genetic early region between genes 1.0 and 1.1, 1 cleavage site Escherichia coli 2 fragments of R1.1 RNA
-
 ?

Subunits

Subunits Comment Organism
More the enzyme contains a conserved dsRNA-binding and a conserved catalytic domain Escherichia coli

Synonyms

Synonyms Comment Organism
More enzyme belongs to the ribonuclease III superfamily Escherichia coli
RNase III
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.023
-
 R1.1 RNA recombinant wild-type enzyme, 37°C, in presence of 1 mM Mn2+ Escherichia coli
0.048
-
 R1.1 RNA recombinant enzyme mutant RNase III[DELTAdsRBD], 37°C, in presence of 5 mM Mn2+ Escherichia coli
0.052
-
 R1.1 RNA recombinant enzyme mutant RNase III[DELTAdsRBD], 37°C, in presence of 25 mM Mg2+ Escherichia coli
0.063
-
 R1.1 RNA recombinant wild-type enzyme, 37°C, in presence of 5 mM Mg2+ Escherichia coli