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Literature summary for 3.1.26.3 extracted from

  • Davidov, Y.; Rahat, A.; Flechner, I.; Pines, O.
    Characterization of the rnc-97 mutation of RNAaseIII: a glycine to glutamate substitution increases the requirement for magnesium ions (1993), J. Gen. Microbiol., 139, 717-724.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Saccharomyces cerevisiae Escherichia coli

Protein Variants

Protein Variants Comment Organism
G97E increases requirement for Mg2+ Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ higher requirement in G97E mtuants Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded RNA + H2O Escherichia coli cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA 5'-phosphooligonucleotides
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of the overexpressed protein in yeast Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded RNA + H2O
-
Escherichia coli 5'-phosphooligonucleotides
-
?
double-stranded RNA + H2O cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA Escherichia coli 5'-phosphooligonucleotides
-
?