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Literature summary for 3.1.26.3 extracted from
- Cleavage of T4 species I ribonucleic acid by Escherichia coli ribonuclease III (1976), Nucleic Acids Res., 3, 1351-1371.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | less than 1 mM | Escherichia coli |  |
| NH4+ | cleavage pattern depends on concentration | Escherichia coli | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | required | Escherichia coli | |
| Co2+ | - |
Escherichia coli | |
| Cu2+ | - |
Escherichia coli | |
| Double-stranded RNA | inhibits cleavage of single-stranded RNA | Escherichia coli | |
| EDTA | greater than 10 mM | Escherichia coli | |
| Fe3+ | - |
Escherichia coli | |
| Hg2+ | - |
Escherichia coli | |
| K+ | - |
Escherichia coli | |
| Mn2+ | - |
Escherichia coli | |
| NaCl | 0.15-0.3 M cleaves at primary cleavage sites, inhibition at higher concentrations, at 5 mM additional secondary cleavage sites | Escherichia coli | |
| NH4+ | concentration dependent cleavage pattern | Escherichia coli | |
| poly(IC) | as double-stranded RNA | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | inhibits | Escherichia coli | |
| Mg2+ | - |
Escherichia coli | |
| Mn2+ | inhibits | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | 55000 | - |
Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded RNA + H2O | Escherichia coli | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | 5'-phosphooligonucleotides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded RNA + H2O | - |
Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
| double-stranded RNA + H2O | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
| single-stranded RNA + H2O | - |
Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
| tRNA + H2O | - |
Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 45 | no activity at 0°C and 17.5°C | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
nonspecific cleavage of RNA after heating for 1 min | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.4 | inactive below, RNA | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | - |
Escherichia coli |
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