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Literature summary for 3.1.26.13 extracted from

  • Julias, J.; McWilliams, M.; Sarafianos, S.; Arnold, E.; Hughes, S.
    Mutations in the RNase H domain of HIV-1 reverse transcriptase affect the initiation of DNA synthesis and the specificity of RNase H cleavage in vivo (2002), Proc. Natl. Acad. Sci. USA, 99, 9515-9520.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
transfection of HEK 293 cells Human immunodeficiency virus 1

Protein Variants

Protein Variants Comment Organism
I505A no discernible effect on viral titer Human immunodeficiency virus 1
K476A mutation reduces the viral titer less than 2fold Human immunodeficiency virus 1
N474A mutation reduces the viral titer less than 2fold Human immunodeficiency virus 1
N474A/Q475A mutation reduces the viral titer 5- to 10fold, , reduction in the efficiency of DNA synthesis. Mutant is less efficient than the wild-type enzyme in its ability to remove a polypurine tract primer from a model substrate and has an altered RNase H cleavage specificity Human immunodeficiency virus 1
Q475A mutation reduces the viral titer 5- to 10fold Human immunodeficiency virus 1
Q500A mutation reduces the viral titer less than 2-fold Human immunodeficiency virus 1
R448A no discernible effect on viral titer Human immunodeficiency virus 1
T473A mutation abolishes viral replication Human immunodeficiency virus 1
Y501A mutation reduces the viral titer 5- to 10fold, reduction in the efficiency of DNA synthesis. Mutant is less efficient than the wild-type enzyme in its ability to remove a polypurine tract primer from a model substrate and has an altered RNase H cleavage specificity Human immunodeficiency virus 1

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information interdependence of the polymerase and RNase H activities of HIV-1 reverse transcription during viral DNA synthesis Human immunodeficiency virus 1 ?
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 ?