Any feedback?
Literature summary for 3.1.26.13 extracted from
- Mutagenesis of cysteine 280 of the reverse transcriptase of human immunodeficiency virus type-1: The effects on the ribonuclease H activity (2003), J. Mol. Biol., 327, 19-30.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C280E | RNase H activity similar to wild-type | Human immunodeficiency virus 1 |
| C280H | RNase H activity similar to wild-type | Human immunodeficiency virus 1 |
| C280L | RNase H activity similar to wild-type | Human immunodeficiency virus 1 |
| C280M | RNase H activity similar to wild-type | Human immunodeficiency virus 1 |
| C280P | significant reduction in RNase H activity. A heterodimer p66C280p/p51C280P shows about 8% of wild-type RNaseH activity, 6% of strand transfer activity, and 105% of DNA polymerase activity. A heterodimer p66C280P/p51 shows about 60% of wild-type RNaseH activity, 80% of strand transfer activity, and 100% of DNA polymerase activity. A heterodimer p66/p51C280W shows about 30% of wild-type RNaseH activity, 6% of strand transfer activity, and 99% of DNA polymerase activity | Human immunodeficiency virus 1 |
| C280Q | RNase H activity similar to wild-type | Human immunodeficiency virus 1 |
| C280R | RNase H activity similar to wild-type | Human immunodeficiency virus 1 |
| C280S | RNase H activity similar to wild-type | Human immunodeficiency virus 1 |
| C280W | significant reduction in RNase H activity. A heterodimer p66C280W/p51C280W shows about 11% of wild-type RNaseH activity, 6% of strand transfer activity, and 100% of DNA polymerase activity. A heterodimer p66C280W/p51 shows about 44% of wild-type RNaseH activity, 80% of strand transfer activity, and 98% of DNA polymerase activity. A heterodimer p66/p51C280W shows about 29% of wild-type RNaseH activity, 7% of strand transfer activity, and 100% of DNA polymerase activity | Human immunodeficiency virus 1 |
| C280Y | RNase H activity similar to wild-type | Human immunodeficiency virus 1 |
| additional information | chemical modifications by thiol-specific reagents of cysteine 280, located in a helix I in the thumb subdomain of the polymerase domain, affect substantially only the RNase H activity | Human immunodeficiency virus 1 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
poly(rA)n-poly(dT)n | 37°C, wild-type p66/p51 | Human immunodeficiency virus 1 | |
| 0.0177 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280P/p51 | Human immunodeficiency virus 1 | |
| 0.0257 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66/p51C280P | Human immunodeficiency virus 1 | |
| 0.0318 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280W/p51 | Human immunodeficiency virus 1 | |
| 0.0383 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66/p51C280W | Human immunodeficiency virus 1 | |
| 0.0553 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280P/p51C280P | Human immunodeficiency virus 1 | |
| 0.0663 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280W/p51C280W | Human immunodeficiency virus 1 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Human immunodeficiency virus 1 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-end-labeled 267 nt-long RNA annealed to 20 nt-long synthetic DNA + H2O | - |
Human immunodeficiency virus 1 | ? | - |
? |  |
| poly(rA)n-poly(dT)n + H2O | - |
Human immunodeficiency virus 1 | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66/p51C280P | Human immunodeficiency virus 1 | |
| 0.024 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66/p51C280W | Human immunodeficiency virus 1 | |
| 0.025 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280P/p51 | Human immunodeficiency virus 1 | |
| 0.029 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280P/p51C280P | Human immunodeficiency virus 1 | |
| 0.029 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280W/p51 | Human immunodeficiency virus 1 | |
| 0.031 | - |
poly(rA)n-poly(dT)n | 37°C, wild-type p66/p51 | Human immunodeficiency virus 1 | |
| 0.034 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280W/p51C280W | Human immunodeficiency virus 1 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 510 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280W/p51C280W | Human immunodeficiency virus 1 | |
| 520 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280P/p51C280P | Human immunodeficiency virus 1 | |
| 620 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66/p51C280W | Human immunodeficiency virus 1 | |
| 910 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280W/p51 | Human immunodeficiency virus 1 | |
| 930 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66/p51C280P | Human immunodeficiency virus 1 | |
| 1460 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280P/p51 | Human immunodeficiency virus 1 | |
| 10300 | - |
poly(rA)n-poly(dT)n | 37°C, wild-type p66/p51 | Human immunodeficiency virus 1 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
