Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.26.13 extracted from

  • Roth, M.J.; Tanese, N.; Goff, S.P.
    Purification and characterization of murine retroviral reverse transcriptase expressed in Escherichia coli (1985), J. Biol. Chem., 260, 9326-9335.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information deletion of 204 nucleotides at the 3'-terminus results in 4fold increase in activity level upon recombinant expression and allows for high-level production of the protein Moloney murine leukemia virus

Inhibitors

Inhibitors Comment Organism Structure
actinomycin D limits the enzyme to the first strand synthesis Moloney murine leukemia virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for synthesis of long DNA strands Moloney murine leukemia virus
Mn2+ required for synthesis of long DNA strands Moloney murine leukemia virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
glycerol gradient centrifugation Moloney murine leukemia virus

Organism

Organism UniProt Comment Textmining
Moloney murine leukemia virus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
166
-
37°C Moloney murine leukemia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A)+ mRNA primed with oligo(dT) + H2O multifunctional enzyme containing RNase H and reverse transcriptase activity Moloney murine leukemia virus double-stranded DNA copies between 1.3 and 9.9 kilobases in length + ?
-
?
RNA:DNA hybrid + H2O
-
Moloney murine leukemia virus AMP + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 71000, SDS-PAGE of recombinant enzyme with deletion of 204 nucleotides at the 3'-terminus Moloney murine leukemia virus