Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of 204 nucleotides at the 3'-terminus results in 4fold increase in activity level upon recombinant expression and allows for high-level production of the protein | Moloney murine leukemia virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
actinomycin D | limits the enzyme to the first strand synthesis | Moloney murine leukemia virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for synthesis of long DNA strands | Moloney murine leukemia virus | |
Mn2+ | required for synthesis of long DNA strands | Moloney murine leukemia virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
glycerol gradient centrifugation | Moloney murine leukemia virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moloney murine leukemia virus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
166 | - |
37°C | Moloney murine leukemia virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(A)+ mRNA primed with oligo(dT) + H2O | multifunctional enzyme containing RNase H and reverse transcriptase activity | Moloney murine leukemia virus | double-stranded DNA copies between 1.3 and 9.9 kilobases in length + ? | - |
? | |
RNA:DNA hybrid + H2O | - |
Moloney murine leukemia virus | AMP + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 71000, SDS-PAGE of recombinant enzyme with deletion of 204 nucleotides at the 3'-terminus | Moloney murine leukemia virus |