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Literature summary for 3.1.25.1 extracted from
- Active site plasticity of endonuclease V from Salmonella typhimurium (2005), Biochemistry, 44, 675-683.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene endo V, expression of wild-type and mutant C-terminally His-tagged enzymes in Escherichia coli strain BL21(DE3) | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D35A | site-directed mutagenesis, the mutant shows no deoxyinosine cleavage activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| Mg2+ | activates | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Mn2+ | activates | Salmonella enterica subsp. enterica serovar Typhimurium | |
| additional information | the enzyme requires a metal ion for activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Salmonella enterica subsp. enterica serovar Typhimurium | the enzyme initiates repair of deaminated bases in damaged DNA by making a nucleolytic incision one nucleotide away from the 3' side of the lesion | ? | - |
? | |
| additional information | Salmonella enterica subsp. enterica serovar Typhimurium 14028 | the enzyme initiates repair of deaminated bases in damaged DNA by making a nucleolytic incision one nucleotide away from the 3' side of the lesion | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
gene endo V | - |
| Salmonella enterica subsp. enterica serovar Typhimurium 14028 | - |
gene endo V | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant C-terminally His-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel chelating affinity chromatography to homogeneity | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme initiates repair of deaminated bases in damaged DNA by making a nucleolytic incision one nucleotide away from the 3' side of the lesion | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| additional information | substrate specificity, the enzyme performs single-turnover on cleavage of double-stranded or single-stranded deoxynucleotide-containing DNA, the enzyme cleaves deoxyinosine- or deoxyxanthosine-containing DNA with similar nicking activity for the substrates, tight binding to nicking products, deoxyuridine-containing DNA is only slightly acepted as substrate when it is in excess, the active site of the enzyme can accomodate pyrimidine-containing mismatches, the plastic nature of the active site allows the enzyme to enfold both pyrimidine and purine deaminated lesions or base pair mismatches, overview | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| additional information | the enzyme initiates repair of deaminated bases in damaged DNA by making a nucleolytic incision one nucleotide away from the 3' side of the lesion | Salmonella enterica subsp. enterica serovar Typhimurium 14028 | ? | - |
? | |
| additional information | substrate specificity, the enzyme performs single-turnover on cleavage of double-stranded or single-stranded deoxynucleotide-containing DNA, the enzyme cleaves deoxyinosine- or deoxyxanthosine-containing DNA with similar nicking activity for the substrates, tight binding to nicking products, deoxyuridine-containing DNA is only slightly acepted as substrate when it is in excess, the active site of the enzyme can accomodate pyrimidine-containing mismatches, the plastic nature of the active site allows the enzyme to enfold both pyrimidine and purine deaminated lesions or base pair mismatches, overview | Salmonella enterica subsp. enterica serovar Typhimurium 14028 | ? | - |
? | |
| oligonucleotide DNA + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| oligonucleotide DNA + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium 14028 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endonuclease V | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| More | the enzyme belongs to the endonuclease V family | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
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