Literature summary for 3.1.25.1 extracted from

Ahn, H.C.; Ohkubo, T.; Iwai, S.; Morikawa, K.; Lee, B.J.
Interaction of T4 endonuclease V with DNA: importance of the flexible loop regions in protein-DNA interaction (2003), J. Biol. Chem., 278, 30985-30992.

Search for more literature for 3.1.25.1

Protein Variants

Protein Variants	Comment	Organism
E23Q	enzyme mutant-substrate complex structure	Tequatrovirus T4

Organism

Organism	UniProt	Comment	Textmining
Tequatrovirus T4	P04418	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate	substrate recognition mechanism: 2 flexible loops are involved in binding and scanning along DNA duplex to search for thymidine dimers within UV-damaged DNA, residues Arg22, Glu23, Thr88 are involved in DNA binding and catalysis, and the regions of Lys45-Phe60 and Lys86-Asp92 are involved in DNA binding and scanning	Tequatrovirus T4	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	excision of pyrimidine dimers produced within duplex DNA by UV radiation, specific for thymine-dimers, interaction with wild-type and duplex DNA, overview	Tequatrovirus T4	?	-	?
additional information	enzyme binds to wild-type and damaged DNA	Tequatrovirus T4	?	-	?

Subunits

Subunits	Comment	Organism
More	determination of secondary structure derived from crystal structure	Tequatrovirus T4

Synonyms

Synonyms	Comment	Organism
T4 Endo V	-	Tequatrovirus T4
T4 endonuclease V	-	Tequatrovirus T4

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)