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Literature summary for 3.1.25.1 extracted from
- Studies on temperature-dependent ultraviolet light-sensitive mutants of bacteriophage T4: the structural gene for T4 endonuclease V (1976), J. Mol. Biol., 102, 15-26.
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | Tequatrovirus T4 | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tequatrovirus T4 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| WT-enzyme: 300fold, enzyme from mutant strain F794: partially purified | Tequatrovirus T4 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00063 | - |
endonuclease from T4 mutant strain F794, partially purified | Tequatrovirus T4 |
| 0.00167 | - |
- |
Tequatrovirus T4 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
7% of activity lost after 10 min | Tequatrovirus T4 |
| 42 | - |
T4 mutant strains: F794 shows 77% inactivation of endonuclease after 2 min whereas F431, F821 and T4D show 7% inactivation after 10 min | Tequatrovirus T4 |
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